CHARACTERISTICS OF MOTOR APPARATUS IN TESTICULAR, EPIDIDYMAL AND EJACULATED SPERMATOZOA - A STUDY USING DEMEMBRANATED SPERM MODELS
CHARACTERISTICS OF MOTOR APPARATUS IN TESTICULAR, EPIDIDYMAL AND EJACULATED SPERMATOZOA - A STUDY USING DEMEMBRANATED SPERM MODELS
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DOI：
10.1016/0014-4827(80)90426-7
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发表时间：
1980-01-01
影响因子：
3.7
通讯作者：
YANAGIMACHI, R
中科院分区：
文献类型：
--
作者：
MOHRI, H;YANAGIMACHI, R
从睾丸和附睾头取出的仓鼠精子实际上是不动的，而附睾尾部和射精的精子能够主动进行运动。获能和活化的精子具有非常旺盛的活力，其特征是其鞭毛像鞭打一样跳动。当这 5 种类型的精子用 Triton X-100 脱膜并暴露于 ATP（和环[c]AMP）时，所有精子都开始主动运动，这表明运动装置甚至在精子离开睾丸之前就已经进行了功能性组装。这些类型的精子中激活的精子百分比以及激活的模式和强度有些不同。成熟（附睾尾和射精）精子的激活效果最好，其次是带头附睾精子。睾丸精子的活性最低。精子的运动器官在附睾运输过程中明显成熟或其特征发生变化。暴露于 ATP（和 cAMP）后，脱膜的附睾尾部（仓鼠和豚鼠）和射出的（仓鼠和人类）精子开始剧烈的鞭打样运动。该运动与膜完整的获能精子在受精时表现出的激活运动非常相似或相同。与获能过程中精子运动装置的特性发生变化不同。新鲜附睾和射精精子中的运动装置必须仅仅通过某些机制来阻止影响激活型鞭毛运动。
Hamster spermatozoa taken from the testis and caput epididymidis are virtually immotile, while cauda epididymal and ejaculated spermatozoa are capable of active progressive movement. Capacitated and activated spermatozoa have very vigorous motility characterized by whiplash-like beating of their flagella. When spermatozoa of these 5 types were demembranated with Triton X-100 and exposed to ATP (and cyclic[c]AMP), all began active movement, suggesting that the motor apparatus was already functionally assembled even before the spermatozoa leave the testis. The percentage of permatozoa activated and the pattern and intensity of activation were somewhat different among these types of spermatozoa. Mature (cauda epididymal and ejaculated) spermatozoa were activated best, followed by the caput epididymal spermatoza. The testicular spermatozoa were least activated. The motor apparatus in the spermatozoon evidently matures or its characteristics change during epididymal transit. Upon exposure to ATP (and cAMP), demembranated cauda epididymal (hamster and guinea pig) and ejaculated (hamster and human) spermatozoa began vigorous whiplash-like movement. The movement was very similar to or identical with the activated movement that membrane-intact capacitated spermatozoa display about the time of fertilization. It is unlike that the characteristics of the sperm motor apparatus changes during capacitation. The motor apparatus in fresh epididymal and ejaculated spermatozoa must be merely prevented by some mechanisms from effecting the activated type of flagellar movement.