HATCHING IN TOAD XENOPUS-LAEVIS - MORPHOLOGICAL EVENTS AND EVIDENCE FOR A HATCHING ENZYME
HATCHING IN TOAD XENOPUS-LAEVIS - MORPHOLOGICAL EVENTS AND EVIDENCE FOR A HATCHING ENZYME
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DOI：
10.1016/0012-1606(74)90254-1
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发表时间：
1974-01-01
影响因子：
2.7
通讯作者：
HEDRICK, JL
中科院分区：
文献类型：
--
作者：
CARROLL, EJ;HEDRICK, JL
非洲爪蟾（Xenopus laevis）胚胎的孵化过程包括两个时间上不同的阶段。在第1阶段，胚胎依次从两个最外面的胶状层J3和J2中逃脱，在第2阶段，胚胎从最后剩下的胶状外套层J1和受精包膜中孵化。第一阶段孵化似乎是一个物理过程所造成的吸水的果冻外套层J1和动态变化的体积封闭的受精信封。组合的膨压使胶状物涂层J3和J2破裂。随后的阶段2孵化是物理和化学过程的结果。阶段1的阴影使层J1暴露于介质中，与凝胶层J2和J3相比，该介质是部分可溶的，并允许其在阶段2期间逐渐溶解。胚胎从额区分泌蛋白水解酶，部分破坏受精膜;随后的胚胎运动使弱化的受精膜破裂，完成孵化过程。
The hatching process in embryos of the toadXenopus laevisconsists of two temporally distinct phases. In phase 1, the embryo escapes sequentially from the two outermost jelly layers, J3and J2, and during phase 2 the embryo hatches from the last remaining jelly coat layer J1and the fertilization envelope. Phase 1 hatching appears to be a physical process caused by water inbibition of jelly coat layer J1and dynamic changes in the volume enclosed by the fertilization envelope. The combined turgor pressure ruptures jelly coat layers J3and J2. The subsequent phase 2 hatching is a result of both physical and chemical processes. Phase 1 hatching exposes layer J1to the medium which, in contrast to jelly layers J2and J3is partially soluble, and permits its gradual dissolution during Phase 2. The embryo secretes a proteolytic enzyme from the frontal region which partially digests the fertilization envelope; subsequent embryo movement ruptures the weakened envelope and completes the hatching process.