Expression and functional characterization of odorant-binding protein genes in the endoparasitic wasp Cotesia vestalis
Expression and functional characterization of odorant-binding protein genes in the endoparasitic wasp Cotesia vestalis
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内寄生黄蜂 Cotesia Vestalis 气味结合蛋白基因的表达和功能特征
DOI：
10.1111/1744-7917.12861
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发表时间：
2020
期刊：
影响因子：
4
通讯作者：
Chen Xue-Xin
中科院分区：
文献类型：
--
作者：
Zhou Yue-Nan;Xie Shuang;Chen Jia-Ni;Wang Ze-Hua;Yang Pei;Zhou Si-Cong;Pang Lan;Li Fei;Shi Min;Huang Jian-Hua;Chen Xue-Xin
气味结合蛋白（OBP）是昆虫嗅觉的关键蛋白，参与气味分子从外界环境向嗅觉受体神经元转运的初始步骤。为了更好地了解OBP在小菜蛾Cotesia vestalis嗅觉感知中的作用，我们对小菜蛾Cotesia vestalis的基因组进行了全面的筛选。vestalis，并获得了20个CvesOBP，包括18个经典OBP和两个minus-C OBP。模体模式分析表明C. vestalisOBP在Hypltera中高度保守。组织表达分析结果表明，5个OBP基因（CvesOBP 1/11/12/14/16）在雄虫触角中高表达，而另外6个OBP基因（CvesOBP 7/8/13/17/18/19）在雌虫触角中转录水平显著富集。对雌蜂触角中3个高表达OBP基因（CvesOBP 17/18/19）的RNA干扰实验结果表明，它们可能参与了雌蜂的寄生过程，因为当它们被击倒时，黄蜂需要更长的时间才能靶向宿主。
Odorant‐binding proteins (OBPs) are crucial in insect's olfactory perception, which participate in the initial step of odorant molecules transporting from the external environment to olfactory receptor neurons. To better understand the roles for OBPs in olfactory perception inCotesia vestalis, a solitary larval endoparasitoid of diamondback moth,Plutella xylostella, we have comprehensively screened the genome ofC. vestalis, and obtained 20 CvesOBPs, including 18 classic OBPs and two minus‐C OBPs. Motif‐pattern analysis indicates that the motifs ofC. vestalisOBPs are highly conserved in Hymenoptera. The results of tissue expression analysis show that five OBPs (CvesOBP1/11/12/14/16) are highly expressed in male antennae, whereas six other OBP genes (CvesOBP7/8/13/17/18/19) are significantly transcriptionally enriched in female antennae. The results of RNA interference experiments for three most highly expressed OBP genes (CvesOBP17/18/19) in female antennae demonstrate that they are likely involved in parasitic processes of female wasps, as the wasps take a longer time to target the hosts when they are knocked down.