Comparison of microglomerular structures in the mushroom body calyx of neopteran insects
Comparison of microglomerular structures in the mushroom body calyx of neopteran insects
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DOI：
10.1016/j.asd.2010.12.002
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发表时间：
2011-07-01
影响因子：
2
通讯作者：
Roessler, Wolfgang
中科院分区：
文献类型：
--
作者：
Groh, Claudia;Roessler, Wolfgang
蘑菇体(MBS)是昆虫大脑中重要的神经鞘，参与感觉整合、学习记忆和空间定向等高级加工过程。不同昆虫的MBS的大小和一般形态是不同的。在这项研究中，我们比较研究了不同种类的新翅目昆虫MBS的主要感觉输入区域的突触复合体(微球)的微观结构。用抗突触素免疫细胞化学、f-肌动蛋白-鬼臼蛋白标记和高分辨率共聚焦显微镜分析微球突触前和突触后的隔室。我们的结果表明，在所有被研究的新翅目昆虫物种中都存在花盏微球，但在突触前和突触后的间隔中，突触素和f-肌动蛋白的分布存在差异。与我们调查的不同昆虫目的所有其他物种相比，膜翅目MBS含有最多的微球数量和堆积密度。我们的结论是，膜翅目中大量微球的进化可能反映了突触微回路的增加，这可能增强了MBS的计算能力。(C)2010爱思唯尔有限公司。保留所有权利。
Mushroom bodies (MBs) are prominent neuropils in the insect brain involved in higher order processing such as sensory integration, learning and memory, and spatial orientation. The size and general morphology of MBs are diverse across insects. In this study we comparatively investigated the microstructure of synaptic complexes (microglomeruli) in major sensory input regions of the MBs, the calyces, across various neopteran insect species. Pre- and postsynaptic compartments of microglomeruli were analyzed using anti-synapsin immunocytochemistry, f-actin-phalloidin labeling and high-resolution confocal microscopy. Our results suggest that calycal microglomeruli are present across all investigated neopteran insect species, but differences are found in the distribution of synapsin and f-actin within their pre- and postsynaptic compartments. Hymenopteran MBs contain the highest number and packing density of microglomeruli compared to all other species from the different insect orders we investigated. We conclude that the evolution of high numbers of microglomeruli in Hymenoptera may reflect an increase in synaptic microcircuits, which could enhance the computational capacities of the MBs. (C) 2010 Elsevier Ltd. All rights reserved.