Differentially expressed genes in multidrug resistant variants of U-2 OS human osteosarcoma cells.
Differentially expressed genes in multidrug resistant variants of U-2 OS human osteosarcoma cells.
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DOI：
10.3892/or.11.6.1257
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发表时间：
2004-06
期刊：
影响因子：
4.2
通讯作者：
T. Chano;K. Mori;K. Scotlandi;S. Benini;C. Lapucci;M. Manara;M. Serra;P. Picci;H. Okabe;N. Baldini
中科院分区：
文献类型：
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作者：
T. Chano;K. Mori;K. Scotlandi;S. Benini;C. Lapucci;M. Manara;M. Serra;P. Picci;H. Okabe;N. Baldini
对抗癌药物的多药耐药性（MDR）是成功治疗人类骨肉瘤的主要障碍。目前对导致 MDR 特征的基因的了解有限，且其机制仍不清楚。在这里，我们将差异显示逆转录聚合酶链反应（DDRT-PCR）应用于U-2 OS人骨肉瘤细胞的亲本和MDR变体，以阐明MDR细胞中涉及的基因，并鉴定了5个候选基因。这些是编码跨膜外排泵的 BCRP（乳腺癌抗性蛋白）； RB1CC1（RB1诱导卷曲螺旋1），一种调节RB1（视网膜母细胞瘤1）表达的肿瘤抑制因子； dUTPase 的新型转录变体； SSR2（β信号序列受体），与跨内质网膜的蛋白质易位相关；和编码高分子量热休克蛋白的HSP105。这些基因的分子和生物学特征将进一步深入了解人类骨肉瘤中 MDR 和肿瘤进展之间的特征。
Multidrug resistance (MDR) to anticancer agents is a major barrier to the successful treatment of human osteosarcomas. Current understanding of the genes that contribute to the features of MDR is limited, and the mechanisms remain unclear. Here we applied differential display reverse transcription-polymerase chain reaction (DDRT-PCR) to parental and MDR-variants of U-2 OS human osteosarcoma cells, to clarify the genes involved in the MDR cells, and identified five candidate genes. These are BCRP (breast cancer resistance protein) encoding a transmembrane efflux pump; RB1CC1 (RB1-inducible coiled-coil 1), a tumor suppressor regulating RB1 (retinoblastoma 1) expression; a novel transcriptional variant of dUTPase; SSR2 (beta-signal sequence receptor), which is associated with protein translocation across ER membrane; and HSP105 encoding high molecular mass heat shock proteins. Molecular and biological characterization of these genes will yield further insight into the features between MDR and tumor progression in human osteosarcomas.