Detection of Melamine in Gluten, Chicken Feed, and Processed Foods Using Surface Enhanced Raman Spectroscopy and HPLC
Detection of Melamine in Gluten, Chicken Feed, and Processed Foods Using Surface Enhanced Raman Spectroscopy and HPLC
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DOI：
10.1111/j.1750-3841.2008.00901.x
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发表时间：
2008-10-01
影响因子：
3.9
通讯作者：
Mustapha, A.
中科院分区：
文献类型：
--
作者：
Lin, M.;He, L.;Mustapha, A.
三聚氰胺是一种富含氮的化学物质，在2007年的宠物和人类食品召回中受到牵连，给食品工业造成了巨大的经济损失。在这项研究中，三聚氰胺的浓度在小麦面筋蛋白，鸡饲料，和加工食品（即蛋糕和面条）的表面增强拉曼光谱（Sers）结合SERS活性基板测量。Sers能快速检测出面筋蛋白中0.1%的三聚氰胺，鸡饲料中0.05%的三聚氰胺，蛋糕中0.05%的三聚氰胺，面条中0.07%的三聚氰胺。建立了偏最小二乘（PLS）模型，用于Sers检测食品中三聚氰胺：R = 0.90，RMSEP = 0.33。此外，Sers结果通过基于简化的FDA方法的HPLC分析进行了验证。与高效液相色谱法相比，表面增强拉曼光谱法具有快速、简便、样品制备量少等优点，但仍能得到满意的定性和定量结果。这些结果表明，这是一个适用的方法，使用Sers筛选食品，消除假定的三聚氰胺污染的样品群体的阴性样品，然后使用HPLC协议验证假定的阳性样品。结合这两种方法可以提供一个更快速和具有成本效益的方法来监测越来越多的进口食品和饲料产品中的三聚氰胺污染。
Melamine, a nitrogen-rich chemical, was implicated in pet and human food recalls in 2007, which caused enormous economic losses to the food industry. In this study, melamine concentration in wheat gluten, chicken feed, and processed foods ( that is, cake and noodle) was measured by surface enhanced Raman spectroscopy (SERS) in combination with SERS-active substrates. SERS was able to rapidly detect 0.1% melamine in wheat gluten, 0.05% in chicken feed, 0.05% in cakes, and 0.07% in noodle, respectively. A partial least squares (PLS) model was established for the quantification of melamine in foods by SERS: R = 0.90, RMSEP = 0.33. In addition, SERS results were verified by HPLC analysis based on a simplified FDA method. Compared with HPLC, the SERS method is much faster and simpler, requires minimum sample preparation, but still yields satisfactory qualitative and quantitative results. These results demonstrate that it is an applicable approach to use SERS to screen foods, eliminate presumptive negative samples of melamine contamination from the sample population, and then verify presumptive positive samples using HPLC protocols. Combining these 2 methods could provide a more rapid and cost-effective way for monitoring melamine contamination in increasingly large numbers of imported foods and feed products.