Three Dimensional Collagen Scaffold Promotes Intrinsic Vascularisation for Tissue Engineering Applications.
Three Dimensional Collagen Scaffold Promotes Intrinsic Vascularisation for Tissue Engineering Applications.
复制标题
DOI：
10.1371/journal.pone.0149799
复制
发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Liu GS
中科院分区：
文献类型：
--
作者：
Chan EC;Kuo SM;Kong AM;Morrison WA;Dusting GJ;Mitchell GM;Lim SY;Liu GS
在这里，我们描述了一种多孔三维胶原支架材料，它支持体外毛细血管形成，并在体内植入时促进血管形成。胶原支架由I型牛胶原合成，具有80 μm的均匀孔径。在体外，与原代人微血管内皮细胞悬浮在人纤维蛋白凝胶接种支架形成CD 31阳性的毛细血管样结构，具有清晰的管腔。在体内，在小鼠皮下植入后，无细胞胶原支架通过宿主新生血管血管化，而支架材料在8周内逐渐降解。胶原蛋白支架，浸渍与人纤维蛋白原凝胶，皮下植入一个封闭的大鼠股血管的室内。从股动脉血管的新生血管芽侵入整个支架，这些降解4周后完全。所得构建体的血管体积大于来自单独植入纤维蛋白原凝胶的腔室的构建体的血管体积（胶原支架中42.7±5.0 μL对单独纤维蛋白原凝胶中22.5±2.3 μL; p<0.05，n = 7）。在相同的模型中，与无细胞胶原支架相比，接种有人脂肪来源的干细胞（ASC）的胶原支架产生了更大的血管体积增加（具有人ASC的胶原支架中的42.9±4.0 μL vs仅胶原支架中的25.7±1.9 μL; p<0.05，n = 4）。总之，这些胶原支架是生物相容的，可以用于生长更健壮的血管化组织工程移植物，提高植入细胞的存活率。这种支架也可以用作血管生成、三维细胞培养以及体内生长因子和细胞递送研究的测定模型。
Here, we describe a porous 3-dimensional collagen scaffold material that supports capillary formation in vitro, and promotes vascularization when implanted in vivo. Collagen scaffolds were synthesized from type I bovine collagen and have a uniform pore size of 80 μm. In vitro, scaffolds seeded with primary human microvascular endothelial cells suspended in human fibrin gel formed CD31 positive capillary-like structures with clear lumens. In vivo, after subcutaneous implantation in mice, cell-free collagen scaffolds were vascularized by host neovessels, whilst a gradual degradation of the scaffold material occurred over 8 weeks. Collagen scaffolds, impregnated with human fibrinogen gel, were implanted subcutaneously inside a chamber enclosing the femoral vessels in rats. Angiogenic sprouts from the femoral vessels invaded throughout the scaffolds and these degraded completely after 4 weeks. Vascular volume of the resulting constructs was greater than the vascular volume of constructs from chambers implanted with fibrinogen gel alone (42.7±5.0 μL in collagen scaffold vs 22.5±2.3 μL in fibrinogen gel alone; p<0.05, n = 7). In the same model, collagen scaffolds seeded with human adipose-derived stem cells (ASCs) produced greater increases in vascular volume than did cell-free collagen scaffolds (42.9±4.0 μL in collagen scaffold with human ASCs vs 25.7±1.9 μL in collagen scaffold alone; p<0.05, n = 4). In summary, these collagen scaffolds are biocompatible and could be used to grow more robust vascularized tissue engineering grafts with improved the survival of implanted cells. Such scaffolds could also be used as an assay model for studies on angiogenesis, 3-dimensional cell culture, and delivery of growth factors and cells in vivo.