The use of barium salts of protected deoxyribonucleoside-3' p-chlorophenyl phosphates for construction of oligonucleotides by the phosphotriester method: high-yield synthesis of dinucleotide blocks.
The use of barium salts of protected deoxyribonucleoside-3' p-chlorophenyl phosphates for construction of oligonucleotides by the phosphotriester method: high-yield synthesis of dinucleotide blocks.
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使用受保护的脱氧核糖核苷-3对氯苯基磷酸的钡盐通过磷酸三酯法构建寡核苷酸：二核苷酸块的高产率合成。
作者：
G. Gough;K. Collier;H. Weith;P. Gilham
通过磷酸三酯法合成多脱氧核糖核苷酸的一种新的实验方法包括直接使用容易制备的O5‘，N-保护的脱氧核糖核苷-3’-氯苯基磷酸盐作为缩合反应中的关键单体来构建寡核苷酸嵌段。已经通过高产率和高纯度地快速合成所有16个全基保护的二核苷酸(式：见正文)(式：见正文)(式：dN‘=dT、dBZC、dBZA或dibG；(式：见正文)证明了该方法。
A new experimental approach to the synthesis of polydeoxyribonucleotides via the phosphotriester method involves construction of oligonucleotide blocks by direct use of the easily prepared barium salts of O5',N-protected deoxyribonucleoside-3' p-chlorophenyl phosphates as the key monomers in condensation reactions. The procedure has been demonstrated by the rapid synthesis in high yield and purity of all sixteen fuly protected dinucleotides (Formula: see text) (where dN' = dT, dbzC, dbzA, or dibG; (Formula: see text) This set of molecules constitutes a "syllabary" for the preparation of defined sequence oligonucleotides.