Effect of estrogen receptor beta on the osteoblastic differentiation function of human periodontal ligament cells
Effect of estrogen receptor beta on the osteoblastic differentiation function of human periodontal ligament cells
复制标题
雌激素受体β对人牙周膜细胞成骨分化功能的影响
DOI：
10.1016/j.archoralbio.2007.12.011
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发表时间：
2008-06-01
影响因子：
3
通讯作者：
Ding, Yin
中科院分区：
文献类型：
--
作者：
Liang, Li;Yu, Ji-feng;Ding, Yin
目的：通过体外检测碱性磷酸酶(ALP)活性和骨钙素(OCN)产量，探讨雌激素受体β(ERβ)对人牙周膜(HPDL)细胞成骨分化功能的影响。设计：采用短干扰RNA(SiRNA)技术抑制hPDL细胞ERβ的表达；ALP试剂盒比色法测定碱性磷酸酶(ALP)活性，骨钙素ELISA法测定OCN含量。结果：雌二醇可明显促进hPDL细胞ALP活性和OCN的生成。结论：ERβ可能在雌激素诱导的PDL细胞成骨分化功能中起重要作用，雌激素主要通过ERβ影响PDL细胞的成骨能力。(C)2008爱思唯尔有限公司。保留所有权利。
Objective: To investigate the effect of estrogen receptor beta (ER beta) on osteoblastic differentiation function of human periodontal ligament (hPDL) cells by measuring the alkaline phosphatase (ALP) activity and the production of osteocalcin (OCN) in vitro.Design: We employed a short interfering RNA (siRNA) technique to inhibit ER beta expression in hPDL cells; the cells were cultured with a saturating concentration of 17 beta-estradiol (10(-7) M). ALP activity was analysed by colorimetric assay using ALP kit and the amount of OCN was assessed by osteocalcin ELISA kit.Results: It was shown that estradiol significantly enhanced the ALP activity and the production of OCN in hPDL cells. However, the ALP activity and the production of OCN in hPDL-siER beta cells were not significantly changed after estradiol treatment.Conclusions: These results indicate that ER beta may play important roles in estrogen-induced effects on osteoblastic differentiation function of PDL cells and estrogen influences the bone formation capacity of PDL cells mainly via ER beta. (C) 2008 Elsevier Ltd. All rights reserved.