Multiple omics analysis reveals the regulation of SIRT5 on mitochondrial function and lipid metabolism during the differentiation of bovine preadipocytes
Multiple omics analysis reveals the regulation of SIRT5 on mitochondrial function and lipid metabolism during the differentiation of bovine preadipocytes
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DOI：
10.1016/j.ygeno.2023.110773
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发表时间：
2023-12-30
期刊：
影响因子：
4.4
通讯作者：
Deng，Weidong
中科院分区：
文献类型：
--
作者：
Hong，Jieyun;Raza，Sayed Haidar Abbas;Deng，Weidong
前脂肪细胞分化是脂肪形成的一个关键阶段，线粒体扮演着不可否认的关键角色。鉴于脂肪形成过程中转录和代谢信号之间的复杂相互作用，通过多组学分析揭示了sirtuin 5（SIRT5）对线粒体功能和脂质代谢的调节。研究结果表明，SIRT 5在促进线粒体生物合成和维持线粒体功能的前脂肪细胞分化过程中起着至关重要的作用。此外，SIRT5通过广泛调节与分化、能量代谢、脂质合成和线粒体功能相关的基因表达来调节许多生物活性物质的代谢水平。最后，SIRT 5通过多种基因调控，抑制三酰甘油（TAG）的积累，提高不饱和脂肪酸的比例和多样性，为线粒体生物合成过程中膜结构的扩展和稳定提供条件。我们的发现为识别与脂肪组织分化和代谢相关的关键功能基因、信号通路和代谢物质提供了基础。
Preadipocyte differentiation represents a critical stage in adipogenesis, with mitochondria playing an undeniable pivotal role. Given the intricate interplay between transcription and metabolic signaling during adipogenesis, the regulation of sirtuin 5 (SIRT5) on mitochondrial function and lipid metabolism was revealed via multiple omics analysis. The findings suggest thatSIRT5plays a crucial role in promoting mitochondrial biosynthesis and maintaining mitochondrial function during preadipocyte differentiation. Moreover,SIRT5modulates the metabolic levels of numerous bioactive substances by extensively regulating genes expression associated with differentiation, energy metabolism, lipid synthesis, and mitochondrial function. Finally,SIRT5was found to suppress triacylglycerols (TAG) accumulation while enhancing the proportion and diversity of unsaturated fatty acids, and providing conditions for the expansion and stability of membrane structure during mitochondrial biosynthesis through numerous gene regulations. Our findings provide a foundation for the identification of crucial functional genes, signaling pathways, and metabolic substances associated with adipose tissue differentiation and metabolism.