Hsp70 and small Hsps are the major heat shock protein members involved in midgut metamorphosis in the common cutworm, Spodoptera litura
Hsp70 and small Hsps are the major heat shock protein members involved in midgut metamorphosis in the common cutworm, Spodoptera litura
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Hsp70 和小 Hsp 是参与斜纹夜蛾中肠变态的主要热休克蛋白成员
DOI：
10.1111/j.1365-2583.2012.01158.x
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发表时间：
2012-10-01
影响因子：
2.6
通讯作者：
Feng, Q-L.
中科院分区：
文献类型：
--
作者：
Gu, J.;Huang, L-X.;Feng, Q-L.
热休克蛋白（Hsps）是一种重要的伴侣蛋白，参与多种信号通路，调节多种生理过程。早期的研究表明，一些热休克蛋白参与了昆虫的发育。然而，很少有研究探讨热休克蛋白的作用，特别是在幼虫-蛹的变态。在本研究中，在斜纹夜蛾转录组中鉴定了49个热刺单基因，并使用基于标签的数字基因表达系统检测了它们在中肠变态过程中的mRNA表达谱。克隆表达水平差异最大的基因，并利用实时定量PCR分析其在6龄幼虫至蛹中肠道的表达模式。研究了这些基因对幼体激素（JH）和20 -羟基蜕皮激素（20E）的响应。结果表明，22个Hsp单基因mRNA水平在中肠变态过程中发生了显著变化。其中，hsp70、hsp20.4和hsp20.8是上调最多的成员，hsp15.9、hsp19.3和hsp22.0是下调最多的成员。进一步研究表明，JH显著上调hsp70、hsp20.4和hsp20.8的表达。此外，20E使hsp20.4和hsp20.8 mRNA水平均略有升高。然而，hsp15.9、hsp19.3和hsp22.0对JH和20E均无反应。这些结果表明，Hsp70和小热休克蛋白（sHsps）可能是斜纹田鼠中肠变态的主要参与者。目前的研究结果为热休克蛋白超家族在昆虫变态中的作用提供了有价值的见解。
Heat shock proteins (Hsps) are important chaperones, which are involved in various signal pathways and regulate lots of physiological processes. Early research suggested that some Hsps are involved in insect development. However, few studies have been carried out to explore the roles of Hsps, especially in larval−pupal metamorphosis. In the present study, 49 Hsp unigenes were identified in the Spodoptera litura transcriptome and their mRNA expression profiles during midgut metamorphosis were examined using a tag‐based digital gene expression system. The genes with the most different levels of expression were then cloned and their expression patterns in midguts from sixth instar larvae to pupae were analysed using real time quantitative PCR. The responses of these genes to juvenile hormone (JH) and 20‐hydroxyecdysone (20E) were also studied. The results showed that the mRNA levels of 22 Hsp unigenes changed significantly during midgut metamorphosis. Amongst these 22 unigenes, hsp70, hsp20.4 and hsp20.8 were the most up‐regulated members, and hsp15.9, hsp19.3 and hsp22.0 were the most down‐regulated ones. Further studies showed that hsp70, hsp20.4 and hsp20.8 were remarkably up‐regulated by JH. In addition, 20E slightly increased the mRNA levels of both hsp20.4 and hsp20.8. However, hsp15.9, hsp19.3 and hsp22.0 did not respond to either JH or 20E. These results indicate that Hsp70 and small Hsps (sHsps) are probably the major players in midgut metamorphosis in S. litura. The current findings provide valuable insights into the roles of the Hsp superfamily in insect metamorphosis.