Quartz crystal microbalance and electrochemical cytosensing on a chitosan/multiwalled carbon nanotubes/Au electrode
Quartz crystal microbalance and electrochemical cytosensing on a chitosan/multiwalled carbon nanotubes/Au electrode
复制标题
壳聚糖/多壁碳纳米管/金电极上的石英晶体微天平和电化学细胞传感。
DOI：
10.1016/j.snb.2008.04.043
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发表时间：
2008-08-28
影响因子：
8.4
通讯作者：
Yao, Shouzhuo
中科院分区：
文献类型：
--
作者：
Jia, Xueen;Tan, Liang;Yao, Shouzhuo
采用压电石英晶体微天平（QCM）监测哺乳动物细胞在壳聚糖（CS）/多壁碳纳米管（MWCNTs）复合修饰金电极上的粘附。利用扫描电子显微镜（SEM）和傅里叶变换红外光谱（FT-IR）对CS/MWCNTs薄膜的形貌和化学性能进行了表征。人乳腺癌细胞（MCF-7）在CS/MWCNTs膜修饰的金表面或网状CS膜修饰的金表面上粘附生长，并通过QCM的谐振频率（Δ f（0））和运动电阻（Δ R-1）的变化来连续监测和显示其过程。MCF-7细胞在QCM Au电极上的附着/铺展过程降低了Δ f（0），同时增加了Δ R-1，这意味着传感器表面上相当复杂的效应（同时存在质量、粘弹性和可能的表面应力负荷）。MTT法检测细胞在两种表面上的贴壁率和增殖活力。通过荧光显微镜证实了电极表面上细胞的存在和状态。循环伏安法和电化学阻抗谱的铁氰化物/亚铁氰化物对细胞粘附前后进行了检查。所有数据表明，细胞粘附和增殖过程中的生物相容性纳米复合材料表面上更有效。基于细胞的生物传感器具有识别和筛选生物活性药物和其他影响细胞形状和附着的生物分子的潜力。(C)2008 Elsevier B. V.保留所有权利。
The piezoelectric quartz crystal microbalance (QCM) was used to monitor the adhesion of mammalian cells on a chitosan (CS)/multiwalled carbon nanotubes (MWCNTs) composite modified gold electrode. The morphology and chemical properties of the CS/MWCNTs film were characterized with scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy. The human breast cancer cells (MCF-7) were adhered to and grown on the CS/MWCNTs film modified gold surface or a net CS film modified gold surface, and the process of which was continuously monitored and displayed by changes of the resonant frequency (Delta f(0)) and the motional resistance (Delta R-1) of the QCM. The attachment/spreading process of the MCF-7 cells on the QCM Au electrode decreased the Delta f(0) and increased the Delta R-1 simultaneously, implying rather complicated effects (simultaneous mass, viscoelasticity and probable surface-stress load) on the sensor surface. The attachment rate and viability of the cells when proliferating on the two surfaces were detected by the MTT assay. The presence and state of cells on the electrode surface were confirmed by the fluorescent microscopy. Cyclic voltammetry and electrochemical impedance spectroscopy of the ferricyanide/ferrocyanide couple were examined before and after the cell adhesion. All data showed that the cell adhesion and proliferation processes were more efficient on the biocompatiable nanocomposite surfaces. The cell-based biosensor has potential for identification and screening of biologically active drugs and other biomolecules affecting cellular shape and attachment. (C) 2008 Elsevier B.V. All rights reserved.