Morphological and structural features of amylose spherocrystals of A-type.
Morphological and structural features of amylose spherocrystals of A-type.
复制标题
A型直链淀粉球晶的形态和结构特征。
DOI：
10.1016/0141-8130(93)90021-d
复制
发表时间：
1993
影响因子：
8.2
通讯作者：
P. Colonna
中科院分区：
文献类型：
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作者：
W. Helbert;H. Chanzy;V. Planchot;A. Buléon;P. Colonna
通过将乙醇与短链直链淀粉的热水溶液混合，然后缓慢冷却至4°C，生长A型直链淀粉球晶体。用扫描电子显微镜和X射线衍射仪对球形晶体进行了观察和分析，球形晶体的直径约为10 μm。它们也被横截面的透射电子显微镜和电子衍射研究。这些技术表明，每个球晶由A型直链淀粉的细长单晶状域从球晶中心辐射的组装体组成。在这些域中的每一个中，直链淀粉的链轴被发现与域的长轴对齐，与观察到的球形晶体的总体正光学双折射一致。球晶相当脆，很容易沿晶畴边界沿着断裂。这可以解释这些结构对α-淀粉酶消化的敏感性。
Amylose spherocrystals of A-type were grown by mixing ethanol with hot aqueous solutions of short chain amylose followed by slow cooling to 4°C. The spherocrystals which had a diameter of the order of 10 μm were observed by scanning electron microscopy and analysed by X-ray diffraction. They were also cross-sectioned for transmission electron microscopy and electron diffraction investigation. These techniques showed that each spherocrystal consisted of an assembly of thin elongated single crystal-like domains of A-type amylose radiating from the centre of the spherocrystal. In each of these domains, the chain axis of amylose was found to be aligned with the long axis of the domain, in agreement with the overall positive optical birefringence observed for the spherocrystals. The spherocrystals which were rather fragile broke easily along the boundaries of the crystalline domains. This could explain the susceptibility of these structures to α-amylase digestion.
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