A simple and effective method for detecting precipitated proteins in MALDI-TOF MS.
A simple and effective method for detecting precipitated proteins in MALDI-TOF MS.
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一种在 MALDI-TOF MS 中检测沉淀蛋白质的简单有效的方法。
DOI：
10.1016/j.ab.2018.01.020
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Nakaki T.
中科院分区：
文献类型：
--
作者：
Oshikane H;Watabe M;Nakaki T.
MALDI-TOF MS已迅速发展成为生命科学的重要分析工具。肉桂酸衍生物通常用于使用MALDI-TOF MS进行完整蛋白质的常规分子量测定。然而，当与基质溶液混合时，感兴趣的蛋白质可能会沉淀，可能会妨碍MS检测。我们在此提供了一种简单的方法，通过“直接沉积法”--将沉淀剂直接加载到样品板上--来实现对这种沉淀的蛋白质物质的MS检测。因此，预期改进完整蛋白质的常规MS分析。
MALDI−TOF MS has developed rapidly into an essential analytical tool for the life sciences. Cinnamic acid derivatives are generally employed in routine molecular weight determinations of intact proteins using MALDI−TOF MS. However, a protein of interest may precipitate when mixed with matrix solution, perhaps preventing MS detection. We herein provide a simple approach to enable the MS detection of such precipitated protein species by means of a “direct deposition method” -- loading the precipitant directly onto the sample plate. It is thus expected to improve routine MS analysis of intact proteins.