Pathogenicity Genomic Island-Associated CrpP-Like Fluoroquinolone-Modifying Enzymes among Pseudomonas aeruginosa Clinical Isolates in Europe
Pathogenicity Genomic Island-Associated CrpP-Like Fluoroquinolone-Modifying Enzymes among Pseudomonas aeruginosa Clinical Isolates in Europe
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DOI：
10.1128/aac.00489-20
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发表时间：
2020-07-01
影响因子：
4.9
通讯作者：
Poirel, Laurent
中科院分区：
文献类型：
--
作者：
de la Rosa, Jose Manuel Ortiz;Nordmann, Patrice;Poirel, Laurent
在革兰氏阴性细菌中已发现许多可转移的喹诺酮耐药机制。最近在铜绿假单胞菌分离株中鉴定出了质粒编码的 65 个氨基酸长的环丙沙星修饰酶 CrpP。我们分析了 100 个克隆无关且多重耐药的铜绿假单胞菌临床分离株，其中 46 个 CrpP 样基因呈阳性，编码 5 个 CrpP 变体，这些变体赋予不同程度的氟喹诺酮类药物敏感性降低。这些 crpP 样基因在染色体上定位为致病性基因组岛的一部分。
Many transferable quinolone resistance mechanisms have been identified in Gram-negative bacteria. The plasmid-encoded 65-amino-acid-long ciprofloxacin-modifying enzyme CrpP was recently identified in Pseudomonas aeruginosa isolates. We analyzed a collection of 100 clonally unrelated and multidrug-resistant P. aerugi-nosa clinical isolates, among which 46 were positive for crpP-like genes, encoding five CrpP variants conferring variable levels of reduced susceptibility to fluoroquino-lones. These crpP-like genes were chromosomally located as part of pathogenicity genomic islands.