Molecular characterization of rare bovine group A rotavirus G15P[11] and G15P[21] strains from eastern India: identification of simian SA11-like VP6 genes in G15P[21] strains
Molecular characterization of rare bovine group A rotavirus G15P[11] and G15P[21] strains from eastern India: identification of simian SA11-like VP6 genes in G15P[21] strains
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DOI：
10.1007/s11262-008-0260-y
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发表时间：
2008-10-01
期刊：
影响因子：
1.6
通讯作者：
Naik, Trailokya N.
中科院分区：
文献类型：
--
作者：
Ghosh, Souvik;Samajdar, Sudipta;Naik, Trailokya N.
在一项监测研究期间(2001年11月至2005年3月)，在印度东部腹泻的牛犊中检测到一种罕见的G15P[11]和两种罕见的G15P[21]牛A组轮状病毒株。对G15P[11]菌株的VP8*、VP6、NSP4和NSP5基因的序列分析证实了其起源于牛。虽然这两个G15P[21]株的NSP4和NSP5基因来自牛，但它们的VP6基因与猿猴株SA11的核苷酸和氨基酸同源性(92.5-93.1%和98.5-98.7%)高于牛株(88.5-88.9%和97-97.2%)，系统发育分析表明，它们与SA11的亲缘关系较远。所有这些都表明，牛和猿猴(SA11-like)株之间可能存在VP6基因重排事件。因此，本研究为G15株的牛来源提供了分子证据，并揭示了牛VP6基因存在罕见的遗传多样性，而在牛A组轮状病毒株中则是保守的。
During a surveillance study (November 2001-March 2005), one rare G15P[11] and two rare G15P[21] bovine group A rotavirus strains were detected in diarrhoeic calves in Eastern India. Sequence analysis of the VP8*, VP6, NSP4 and NSP5 genes of the G15P[11] strain confirmed its bovine origin. Although the NSP4 and NSP5 genes of the two G15P[21] strains were of bovine origin, their VP6 genes shared higher nucleotide and amino acid identities with simian strain SA11 (92.5-93.1% and 98.5-98.7%) than bovine strains (88.5-88.9% and 97-97.2%), and by phylogenetic analysis, exhibited clustering with SA11, distantly related to bovine strains. All these pointed towards a possible reassortment event of VP6 gene between bovine and simian (SA11-like) strains. Therefore, the present study provided molecular evidence for bovine origin of G15 strains and revealed a rare instance of genetic diversity in the bovine VP6 gene, otherwise conserved in group A rotavirus strains from cattle.