Microbial diversity in sediments collected from the deepest cold-seep area, the Japan Trench
Microbial diversity in sediments collected from the deepest cold-seep area, the Japan Trench
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DOI：
10.1007/pl00011793
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发表时间：
1999-07-01
影响因子：
3
通讯作者：
Horikoshi, K
中科院分区：
文献类型：
--
作者：
Li, L;Kato, C;Horikoshi, K
日本海沟陆坡6,400 m深处是最深的冷泉环境，有Calyptogena群落。采集了Calyptogena群落内和群落旁的沉积物样品，采用分子系统发育学方法对沉积物样品中微生物多样性进行了研究。直接从沉积物样品中提取DNA，通过聚合酶链反应方法扩增16 S rDNA。通过限制性片段长度多态性分析选择扩增的16 S rDNA序列进行测定，并与DNA数据库中的序列进行比较。结果表明，33个不同的细菌16 S rDNA序列分析的两个样品属于相似的系统发育类别，α，γ，δ和ε-变形菌，噬纤维菌和革兰氏阳性细菌的细分;一些16 S rDNA序列是共同的两个样品。三角洲和ε-变形菌相关序列丰富的沉积物。这些序列主要分别与硫酸盐还原菌或硫还原菌和表生生物有关。从沉积物中克隆了8个不同的古菌16 S rDNA序列。大多数古菌16 S rDNA序列聚在泉古菌群中，与海洋I组古菌rDNA具有很高的相似性。从广古菌群的沉积物中获得的一个与Methanocococcoides burtonii相关的序列表明M.在Calyptogena群落区域中的burtonii相关菌株可能有助于在该环境中产生甲烷。从这些结果，我们提出了一个可能的模式硫循环内的微生物群落和Calyptogena蛤在冷泉环境。
The Japan Trench land slope at a depth of 6,400 m is the deepest cold-seep environment with Calyptogena communities. Sediment samples from inside and beside the Calyptogena communities were collected, and the microbial diversity in the sediment samples was studied by molecular phylogenetic techniques. From DNA extracted directly from the sediment samples, 16S rDNAs were amplified by the polymerase chain reaction method. The sequences of the amplified 16S rDNAs selected by restriction fragment length polymorphism analysis were determined and compared with sequences in DNA databases. The results showed that 33 different bacterial 16S rDNA sequences from the two samples analyzed fell into similar phylogenetic categories, the alpha-, gamma-, delta-, and epsilon-subdivisions of Proteobacteria, Cytophaga, and gram-positive bacteria; some of the 16S rDNA sequences were common to both samples. delta- and epsilon-Proteobacteria-related sequences were abundant in both sediments. These sequences are mostly related to sulfate-reducing or sulfur-reducing bacteria and epibionts, respectively. Eight different archaeal 16S rDNA sequences were cloned from the sediments. The majority of the archaeal 16S rDNA sequences clustered in Crenarchaeota and showed high similarities to marine group I archaeal rDNA. A Methanococcoides burtonii-related sequence obtained from the sediment clustered in the Euryarchaeota indicating that M. burtonii-related strains in the area of Calyptogena communities may contribute to production of methane in this environment. From these results, we propose a possible model of sulfur circulation within the microbial community and that of Calyptogena clams in the cold-seep environment.