Overexpression of aurora-A contributes to malignant development of human esophageal squamous cell carcinoma
Overexpression of aurora-A contributes to malignant development of human esophageal squamous cell carcinoma
复制标题
DOI：
10.1158/1078-0432.ccr-04-0806
复制
发表时间：
2004-11-01
影响因子：
11.5
通讯作者：
Zhan, QM
中科院分区：
文献类型：
--
作者：
Tong, T;Zhong, YL;Zhan, QM
Aurora-A/STK 15/BTAK是一种中心体相关的致癌蛋白，参与有丝分裂的调控。Aurora-A的过表达已显示导致染色体畸变和基因组不稳定性。多种证据表明Aurora-A诱导细胞恶性转化。在当前的研究中，我们有兴趣研究Aurora-A在人食管鳞状细胞癌（ESCC）中的表达，并表征Aurora-A与ESCC恶性进展的相关性。实验设计：通过免疫组织化学或Western印迹分析检测84例ESCC组织和81例配对正常癌旁组织中Aurora-A蛋白的表达。此外，基因敲低小干扰RNA技术被用于在ESCC细胞中，以调查是否极光-A有助于肿瘤的生长invasively.Results的能力：极光-A蛋白在ESCC中的量是相当高的，比在正常的相邻组织。Aurora-A在84例食管鳞癌中有57例（67.5%）表达阳性。与此相反的是，
Purpose: Aurora-A/STK15/BTAK, a centrosome-associated oncogenic protein, is implicated in the control of mitosis. Overexpression of Aurora-A has been shown to result in chromosomal aberration and genomic instability. Multiple lines of evidence indicate that Aurora-A induces cell malignant transformation. In the current study, we are interested in investigating the expression of Aurora-A in human esophageal squamous cell carcinoma (ESCC) and characterizing the association of Aurora-A with ESCC-malignant progression.Experimental Design: Aurora-A protein expression was examined in 84 ESCC tissues and 81 paired normal adjacent tissues by either immunohistochemistry or Western blot analysis. In addition, a gene-knockdown small interfering RNA technique was used in ESCC cells to investigate whether Aurora-A contributes to the ability of a tumor to grow invasively.Results: The amount of Aurora-A protein in ESCC was considerably higher than that in normal adjacent tissues. Overexpression of Aurora-A was observed in 57 of 84 (67.5%) ESCC samples. In contrast,