Association between Mitochondrial DNA Sequence Variants and V˙O2 max Trainability.
Association between Mitochondrial DNA Sequence Variants and V˙O2 max Trainability.
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DOI：
10.1249/mss.0000000000002390
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发表时间：
2020-11
影响因子：
4.1
通讯作者：
Kleeberger, Steven R.
中科院分区：
文献类型：
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作者：
Vellers, Heather L.;Verhein, Kirsten C.;Burkholder, Adam B.;Lee, Jaehoon;Kim, Youngmin;Lightfoot, J. Timothy;Shi, Min;Weinberg, Clarice R.;Sarzynski, Mark A.;Bouchard, Claude;Kleeberger, Steven R.
We designed the study to determine whether mitochondrial DNA (mtDNA) haplogroup, sequence, and heteroplasmy differed between individuals previously characterized as low- (LR) or high-responders (HR) as defined by their VO2 max response to a standardized aerobic exercise training program. DNA was isolated from whole blood in subjects from the HERITAGE Family Study that were determined to be either HR (n=15) or LR (n=15). mtDNA was amplified by long-range polymerase chain reaction, then tagged with Nextera libraries and sequenced on a MiSeq instrument. Different mtDNA haplogroup subtypes were found in HR and LR individuals. Compared to HR subjects, significantly more LR subjects had variants in 13 sites, including seven in hypervariable (HV) regions: HV2 (G185A: 0 vs 6, p = 0.02; G228A: 0 vs 5, p = 0.04; C295T: 0 vs 6; p = 0.04), HV3 (C462T: 0 vs 5, p = 0.04; T489C: 0 vs 5; p = 0.04), and HV1 (C16068T: 0 vs 6, p = 0.02; T16125C: 0 vs 6, p = 0.02). Remaining variants were in protein coding genes, mtND1 (1 vs 8, p = 0.02), mtND3 (A10397G: 0 vs 5, p = 0.04), mtND4 (A11250G: 1 vs 8, p = 0.02), mtND5 (G13707A: 0 vs 5, p = 0.04), and mtCYTB (T14797C: 0 vs 5, p = 0.04; C15451A: 1 vs 8, p = 0.02). Average total numbers of heteroplasmies (p = 0.83) and frequency of heteroplasmies (p = 0.05) were similar between the groups. Our findings provide specific sites across the mitochondrial genome that may be related to VO2 max trainability.
