Anticancer potential of an exopolysaccharide from Lactobacillus helveticus MB2-1 on human colon cancer HT-29 cells via apoptosis induction
Anticancer potential of an exopolysaccharide from Lactobacillus helveticus MB2-1 on human colon cancer HT-29 cells via apoptosis induction
复制标题
瑞士乳杆菌 MB2-1 胞外多糖通过诱导细胞凋亡对人结肠癌 HT-29 细胞的抗癌潜力
DOI：
10.1039/d0fo01345e
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发表时间：
2020
期刊：
影响因子：
6.1
通讯作者：
Li Wei
中科院分区：
文献类型：
--
作者：
Xiao Luyao;Ge Xiaojia;Yang Lin;Chen Xiaohong;Xu Qian;Rui Xin;Fan Xia;Feng Li;Zhang Qiuqin;Dong Mingsheng;Li Wei
本研究旨在研究从瑞士乳杆菌MB2-1中分离的胞外多糖(EPS)的抗癌活性。将Helveticus MB2-1(LHEPS)的EPS粗品分为三个组分，即LHEPS-1、LHEPS-2和LHEPS-3。LHEPS-1显示出最有效的抗增殖活性，这与其对人结肠癌HT-29细胞的抑制率和乳酸脱氢酶漏出增加有关。流式细胞仪和比色分析显示，LHEPS-1通过阻止G(1)向S的转变而诱导细胞周期停滞，并增加细胞凋亡率。此外，LHEPS-1促进细胞内ROS的产生和caspase-8/9/3的活性，增加促凋亡的Bax和线粒体细胞色素c的水平，而降低抗凋亡的Bcl2的水平，提示LHEPS-1可能通过ROS依赖和线粒体依赖的途径诱导HT-29细胞的凋亡。这些发现表明，LHEPS-1可能被开发为一种有效的食物和/或药物来预防和治疗癌症，特别是人类结肠癌。
This study aimed at investigating the anticancer activity of an exopolysaccharide (EPS) isolated from Lactobacillus helveticus MB2-1. The crude EPS from L. helveticus MB2-1 (LHEPS) was fractionated into three fractions, namely LHEPS-1, LHEPS-2 and LHEPS-3. LHEPS-1 exhibited the most effective anti-proliferative activity, which was associated with a stronger inhibition rate and increased lactate dehydrogenase leakage of human colon cancer HT-29 cells. Flow cytometry analysis and colorimetric assay revealed that LHEPS-1 induced cell cycle arrest by preventing G(1) to S transition and increased the apoptosis rate. Furthermore, LHEPS-1 enhanced the production of intracellular reactive oxygen species (ROS) and the activity of caspases-8/9/3, increased the levels of pro-apoptotic Bax and mitochondrial cytochrome c, while decreased the anti-apoptotic Bcl-2 level, indicating that LHEPS-1 might induce the apoptosis of HT-29 cells through a ROS-dependent pathway and a mitochondria-dependent pathway. These findings suggest that LHEPS-1 may be developed as an effective food and/or drug for the prevention and therapeutics of cancer, especially human colon cancer.