PROPAGATION OF THE VIRUS OF PORCINE EPIDEMIC DIARRHEA IN CELL-CULTURE
PROPAGATION OF THE VIRUS OF PORCINE EPIDEMIC DIARRHEA IN CELL-CULTURE
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DOI：
10.1128/jcm.26.11.2235-2239.1988
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发表时间：
1988-11-01
影响因子：
9.4
通讯作者：
WYLER, R
中科院分区：
文献类型：
--
作者：
HOFMANN, M;WYLER, R
通过在培养基中添加胰蛋白酶，使猪流行性腹泻病毒（PEDV）适应在 Vero 细胞培养物中的连续繁殖。通过荧光抗体染色技术检查时，PEDV 感染的细胞显示出独特的细胞质荧光。即使在 Vero 细胞中 PEDV 的第 1 代，也检测到了细胞病变效应，例如空泡形成、合胞体形成和细胞融合。一旦适应，病毒就会诱导产生大量含有 100 多个细胞核的合胞体。从病毒第5代开始，所有形成单层的细胞在接种后24小时内融合并完全破坏。通过电子显微镜观察，细胞培养物生长的 PEDV 具有典型的冠状病毒形态。在存在或不存在胰蛋白酶的情况下在几种原代和继代胎猪细胞培养物中繁殖PEDV的尝试均未成功。
Porcine epidemic diarrhea virus (PEDV) was adapted to serial propagation in Vero cell cultures by adding trypsin of the medium. PEDV-infected cells showed a distinct cytoplasmic fluorescence when examined by a fluorescent-antibody-staining technique. Cytopathic effects, such as vacuolation, formation of syncytia, and fusion of cells, were detected even at passage 1 of the PEDV in Vero cells. Once adapted, the virus induced numerous syncytia containing over 100 nuclei. From virus passage 5, all cells forming the monolayer were fused and totally destroyed within 24 h after inoculation. Cell culture-grown PEDV had typical coronavirus morphology when viewed by electron microscopy. Attempts to propagate PEDV in several primary and secondary fetal porcine cell cultures in the presence or absence of trypsin were unsuccessful.