GelJ--a tool for analyzing DNA fingerprint gel images.
GelJ--a tool for analyzing DNA fingerprint gel images.
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DOI：
10.1186/s12859-015-0703-0
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发表时间：
2015-08-26
影响因子：
3
通讯作者：
Zarazaga M
中科院分区：
文献类型：
--
作者：
Heras J;Domínguez C;Mata E;Pascual V;Lozano C;Torres C;Zarazaga M
DNA 指纹识别是一种比较 DNA 模式的技术，在多种情况下都有应用。多种商业和免费工具可用于分析 DNA 指纹凝胶图像；然而，商业工具价格昂贵且通常难以使用；而且，免费工具支持 DNA 指纹分析的基本功能，但缺乏一些获得准确结果的工具功能。在本文中，我们介绍了 GelJ，一种轻量级、用户友好、独立于平台、开源且免费的工具，用于分析 DNA 指纹凝胶图像。 GelJ 的一些突出功能包括精确的泳道和条带检测机制、计算迁移模型的多种选项、许多基于条带和曲线的相似性方法、生成树状图的不同技术、不同实验的条带模式的比较以及数据库支持。 GelJ 是一款易于使用的工具，用于分析 DNA 指纹凝胶图像。它结合了免费和商业工具的最佳特性：GelJ 轻便且易于使用（作为免费程序），但它还包含获得精确结果的必要功能（作为商业程序）。此外，GelJ 还集成了任何其他工具都不支持的新功能。本文的在线版本 (doi:10.1186/s12859-015-0703-0) 包含补充材料，可供授权用户使用。
DNA fingerprinting is a technique for comparing DNA patterns that has applications in a wide variety of contexts. Several commercial and freely-available tools can be used to analyze DNA fingerprint gel images; however, commercial tools are expensive and usually difficult to use; and, free tools support the basic functionality for DNA fingerprint analysis, but lack some instrumental features to obtain accurate results. In this paper, we present GelJ, a feather-weight, user-friendly, platform-independent, open-source and free tool for analyzing DNA fingerprint gel images. Some of the outstanding features of GelJ are mechanisms for accurate lane- and band-detection, several options for computing migration models, a number of band- and curve-based similarity methods, different techniques for generating dendrograms, comparison of banding patterns from different experiments, and database support. GelJ is an easy to use tool for analyzing DNA fingerprint gel images. It combines the best characteristics of both free and commercial tools: GelJ is light and simple to use (as free programs), but it also includes the necessary features to obtain precise results (as commercial programs). In addition, GelJ incorporates new functionality that is not supported by any other tool. The online version of this article (doi:10.1186/s12859-015-0703-0) contains supplementary material, which is available to authorized users.