Genome-wide transcriptional changes of ramie (Boehmeria nivea L. Gaud) in response to root-lesion nematode infection.
Genome-wide transcriptional changes of ramie (Boehmeria nivea L. Gaud) in response to root-lesion nematode infection.
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DOI：
10.1016/j.gene.2014.09.014
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发表时间：
2014-11
期刊：
影响因子：
3.5
通讯作者：
Siyuan Zhu;S. Tang;Q. Tang;Toumin Liu
中科院分区：
文献类型：
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作者：
Siyuan Zhu;S. Tang;Q. Tang;Toumin Liu
苎麻纤维是从苎麻茎皮中提取的一种重要的天然纤维。根病线虫是苎麻的主要害虫，每年造成我国苎麻产量的巨大损失。苎麻对RLN感染的反应机制尚不清楚。在这项研究中，我们确定了基因，可能涉及的RLN抗性苎麻使用Illumina对端测序在两个RLN感染的植物（Inf 1和Inf 2）和两个对照植物（CO 1和CO2）。从CO 1、CO2、Inf 1和Inf 2文库分别生成约5630万、5170万、4340万和4500万测序读段。这1.96亿个读段的从头组装产生了50，486个单基因，平均长度为853.3 bp。共有24，820个（49.2%）基因被注释了它们的功能。比较CO和Inf苎麻的基因表达水平，发现777个差异表达基因（DEG）。通过实时定量PCR（qRT-PCR）进一步证实12个DEG的表达水平。途径富集分析表明，三个途径（苯丙氨酸代谢，类胡萝卜素的生物合成，苯丙素的生物合成）的强烈影响RLN感染。一系列可能参与苎麻对RLN防御反应的候选基因和途径将有助于进一步提高苎麻对RLN的抗性。
Ramie fiber extracted from stem bark is one of the most important natural fibers. The root-lesion nematode (RLN)Pratylenchuscoffeaeis a major ramie pest and causes large fiber yield losses in China annually. The response mechanism of ramie to RLN infection is poorly understood. In this study, we identified genes that are potentially involved in the RLN-resistance in ramie using Illumina pair-end sequencing in two RLN-infected plants (Inf1 and Inf2) and two control plants (CO1 and CO2). Approximately 56.3, 51.7, 43.4, and 45.0 million sequencing reads were generated from the libraries of CO1, CO2, Inf1, and Inf2, respectively. De novo assembly for these 196 million reads yielded 50,486 unigenes with an average length of 853.3 bp. A total of 24,820 (49.2%) genes were annotated for their function. Comparison of gene expression levels between CO and Inf ramie revealed 777 differentially expressed genes (DEGs). The expression levels of 12 DEGs were further confirmed by real-time quantitative PCR (qRT-PCR). Pathway enrichment analysis showed that three pathways (phenylalanine metabolism, carotenoid biosynthesis, and phenylpropanoid biosynthesis) were strongly influenced by RLN infection. A series of candidate genes and pathways that may contribute to the defense response against RLN in ramie will be helpful for further improving resistance to RLN infection.