Nestin Positive Bone Marrow Derived Cells Responded to Injury Mobilize into Peripheral Circulation and Participate in Skin Defect Healing.
Nestin Positive Bone Marrow Derived Cells Responded to Injury Mobilize into Peripheral Circulation and Participate in Skin Defect Healing.
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巢蛋白阳性骨髓衍生细胞对损伤有反应，动员进入外周循环并参与皮肤缺损愈合
DOI：
10.1371/journal.pone.0143368
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Deng Z
中科院分区：
文献类型：
--
作者：
Yang Y;Pang D;Hu C;Lv Y;He T;An Y;Tang Z;Deng Z
外源性间充质干细胞（MSCs）被认为可以通过外周血流迁移到损伤部位，参与组织修复。然而，内源性骨髓间充质干细胞是否以及如何动员到循环中并靶向组织损伤，一直存在争议，且由于体内鉴定困难，相关研究受到限制。Nestin是一种最初在神经上皮干细胞中发现的中间丝蛋白，最近被报道为骨髓间充质干细胞的可靠标准。本研究采用绿色荧光蛋白（GFP）标记的骨髓替代模型，追踪皮肤缺损小鼠nestin阳性的骨髓来源细胞（BMDCs）。结果发现，皮肤损伤后，外周血和骨髓中nestin+细胞数量均增加。在皮肤创伤周围可检测到大量的nestin+ BMDCs，而在未损伤的皮肤或其他器官中可观察到少量的这些细胞。粒细胞集落刺激因子（G-CSF）不能促进这种募集作用，提示G-CSF对造血细胞的动员机制与G-CSF不同。我们的研究结果表明，nestin+ BMDCs作为动员的候选人在皮肤损伤修复，这提供了一个新的见解内源性骨髓间充质干细胞治疗。
Exogenously infused mesenchymal stem cells (MSCs) are thought to migrate to injury site through peripheral blood stream and participate in tissue repair. However, whether and how endogenous bone marrow MSCs mobilized to circulating and targeted to tissue injury has raised some controversy, and related studies were restricted by the difficulty of MSCs identifying in vivo. Nestin, a kind of intermediate filament protein initially identified in neuroepithelial stem cells, was recently reported as a credible criteria for MSCs in bone marrow. In this study, we used a green fluorescent protein (GFP) labeled bone marrow replacement model to trace the nestin positive bone marrow derived cells (BMDCs) of skin defected-mice. We found that after skin injured, numbers of nestin+ cells in peripheral blood and bone marrow both increased. A remarkable concentration of nestin+ BMDCs around skin wound was detected, while few of these cells could be observed in uninjured skin or other organs. This recruitment effect could not be promoted by granulocyte colony-stimulating factor (G-CSF), suggests a different mobilization mechanism from ones G-CSF takes effect on hematopoietic cells. Our results proposed nestin+ BMDCs as mobilized candidates in skin injury repair, which provide a new insight of endogenous MSCs therapy.