Evidence of apoptotic cell death after experimental traumatic brain injury in the rat.
Evidence of apoptotic cell death after experimental traumatic brain injury in the rat.
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发表时间：
1995
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通讯作者：
A. Rink;K. Fung;J. Trojanowski;V. Lee;E. Neugebauer;Tracy K. McIntosh
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作者：
A. Rink;K. Fung;J. Trojanowski;V. Lee;E. Neugebauer;Tracy K. McIntosh
细胞凋亡在中枢神经系统的许多发育和病理过程中发挥着重要作用。然而，细胞凋亡在创伤性脑损伤中的作用尚未确定。利用末端脱氧核苷酸转移酶介导的生物素化脱氧尿苷三磷酸缺口末端标记（TUNEL）方法，我们在遭受实验性脑损伤的大鼠大脑的不同区域中检测到许多具有广泛DNA碎片的细胞。通过光学和电子显微镜证实了两种类型的TUNEL阳性细胞，包括表现出坏死性细胞死亡形态学特征的I型细胞和表现出典型细胞凋亡性细胞死亡形态学特征的II型细胞。初次损伤后 72 小时内可检测到 TUNEL 阳性细胞。从含有 I 型和 II 型细胞的受损大脑受影响区域提取的 DNA 进行凝胶电泳，仅显示 185 bp 间隔的核小体间断裂，这一特征最初在细胞凋亡中描述。这些数据表明，除了坏死性细胞死亡之外，外伤性脑损伤后还发生细胞凋亡，并且 DNA 核小体间断裂可能与某些类型的坏死性细胞死亡有关。
Apoptosis plays an important role in many developmental and pathological processes of the central nervous system. However, the role of apoptosis in traumatic brain injury has not been determined. Using the terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nick end labeling (TUNEL) method, we detected many cells with extensive DNA fragmentation in different regions of the brains of rats subjected to experimental traumatic brain injury. Two types of TUNEL-positive cells were demonstrated by light and electron microscopy, including type I cells that displayed morphological features of necrotic cell death and type II cells that displayed morphological features of classic apoptotic cell death. TUNEL-positive cells were detectable for up to 72 hours after the initial injury. Gel electrophoresis of DNA extracted from affected areas of the injured brain containing both type I and II cells revealed only internucleosomal fragmentation at 185-bp intervals, a feature originally described in apoptotic cell death. These data suggest that apoptosis, in addition to necrotic cell death, occurs after traumatic brain injury, and that internucleosomal fragmentation of DNA may be associated with certain types of necrotic cell death.