The zebrafish fgf24 mutant identifies an additional level of Fgf signaling involved in vertebrate forelimb initiation
The zebrafish fgf24 mutant identifies an additional level of Fgf signaling involved in vertebrate forelimb initiation
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DOI：
10.1242/dev.00537
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发表时间：
2003-08-01
期刊：
影响因子：
4.6
通讯作者：
Neumann, CJ
中科院分区：
文献类型：
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作者：
Fischer, S;Draper, BW;Neumann, CJ
脊椎动物肢芽的发育是由侧板中胚层中的一系列基因触发的，包括Fgf和Wnt家族的成员以及转录因子tbx 5。在中间中胚层中表达的fgf 8被认为通过激活wnt 2b启动前肢形成，然后wnt 2b诱导tbx 5在相邻侧板中胚层中的表达。反过来，tbx 5是激活fgf 10所必需的，fgf 10将肢体诱导信号传递到覆盖的外胚层。我们发现，斑马鱼的FGF 24基因，它属于FGF 8/17/18亚家族的FGF配体，行为的TBX 5的下游激活FGF 10的表达在侧板中胚层。我们还表明，fgf 24的活性是必要的迁移tbx 5表达细胞的鳍芽，并激活shh，但不是hand 2，在后鳍芽的表达。
The development of vertebrate limb buds is triggered in the lateral plate mesoderm by a cascade of genes, including members of the Fgf and Wnt families, as well as the transcription factor tbx5. Fgf8, which is expressed in the intermediate mesoderm, is thought to initiate forelimb formation by activating wnt2b, which then induces the expression of tbx5 in the adjacent lateral plate mesoderm. Tbx5, in turn, is required for the activation of fgf10, which relays the limb inducing signal to the overlying ectoderm. We show that the zebrafish fgf24 gene, which belongs to the Fgf8/17/18 subfamily of Fgf ligands, acts downstream of tbx5 to activate fgf10 expression in the lateral plate mesoderm. We also show that fgf24 activity is necessary for the migration of tbx5-expressing cells to the fin bud, and for the activation of shh, but not hand2, expression in the posterior fin bud.