Meloxicam inhibits the growth of colorectal cancer cells
Meloxicam inhibits the growth of colorectal cancer cells
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DOI：
10.1093/carcin/19.12.2195
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发表时间：
1998-12-01
期刊：
影响因子：
4.7
通讯作者：
DuBois, RN
中科院分区：
文献类型：
--
作者：
Goldman, AP;Williams, CS;DuBois, RN
环氧合酶-2在结直肠癌的发生发展中起重要作用。评价了美洛昔康（一种考克斯-2抑制剂）对两种表达考克斯-2的结肠癌细胞系（HCA-7和Moser-S）和一种考克斯-2阴性细胞系（HCT-116）生长的影响。用美洛昔康处理后，测量这些细胞的生长速率。美洛昔康处理后，HCA-7和Moser-S集落大小显著降低;然而，HCT-116集落大小在处理后无显著变化。进行了体内研究，以评价美洛昔康对异种移植到裸鼠体内的HCA-7细胞生长的影响。治疗4周后，我们观察到肿瘤尺寸缩小了51%。HCA-7肿瘤裂解物中考克斯-1和考克斯-2蛋白水平的分析显示，从接受美洛昔康给药的小鼠中采集的肿瘤中考克斯-2表达水平轻微降低，未检测到考克斯-1表达。在这里，我们报告美洛昔康显着抑制HCA-7集落和肿瘤生长，但对考克斯-2阴性HCT-116细胞的生长没有影响。
Cyclooxygenase-2 has been reported to play an important role in colorectal carcinogenesis. The effects of meloxicam (a COX-2 inhibitor) on the growth of two colon cancer cell lines that express COX-2 (HCA-7 and Moser-S) and a COX-2 negative cell line (HCT-116) were evaluated. The growth rate of these cells was measured following treatment with meloxicam. HCA-7 and Moser-S colony size were significantly reduced following treatment with meloxicam; however, there was no significant change in HCT-116 colony size with treatment, lit vivo studies were performed to evaluate the effect of meloxicam on the growth of HCA-7 cells when xenografted into nude mice. We observed a 51% reduction in tumor size after 4 weeks of treatment. Analysis of COX-1 and COX-2 protein levels in HCA-7 tumor lysates revealed a slight decrease in COX-2 expression levels in tumors taken from mice treated with meloxicam and no detectable COX-1 expression. Here we report that meloxicam significantly inhibited HCA-7 colony and tumor growth but had no effect on the growth of the COX-2 negative HCT-116 cells.