Identification of Synthetic Inducers and Inhibitors of the Quorum-Sensing Regulator LasR in Pseudomonas aeruginosa by High-Throughput Screening
Identification of Synthetic Inducers and Inhibitors of the Quorum-Sensing Regulator LasR in Pseudomonas aeruginosa by High-Throughput Screening
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DOI：
10.1128/aem.00499-10
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发表时间：
2010-12-01
影响因子：
4.4
通讯作者：
Handelsman, Jo
中科院分区：
文献类型：
--
作者：
Borlee, Bradley R.;Geske, Grant D.;Handelsman, Jo
我们报道了用激光转录激活剂对恶臭假单胞菌n -酰化l -高丝氨酸内酯（AHL）传感器菌株进行诱导和抑制群体感应的16,000个合成化合物的筛选。激光辐射控制机会致病菌铜绿假单胞菌的毒力基因表达，是一种重要的治疗靶点。在我们的高通量筛选中鉴定出9种抑制和14种诱导LasR活性的化合物。
We report the screening of 16,000 synthetic compounds for induction and inhibition of quorum sensing in a Pseudomonas putida N-acylated L-homoserine lactone (AHL) sensor strain engineered with the LasR transcriptional activator. LasR controls virulence gene expression in the opportunistic pathogen Pseudomonas aeruginosa and is of significant interest as a therapeutic target. Nine compounds that inhibit and 14 compounds that induce LasR activity were identified in our high-throughput screen.