Interkingdom interactions on the denture surface: Implications for oral hygiene
Interkingdom interactions on the denture surface: Implications for oral hygiene
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DOI：
10.1016/j.bioflm.2019.10002
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发表时间：
2019-12-01
期刊：
影响因子：
6.8
通讯作者：
Ramage, Gordon
中科院分区：
文献类型：
--
作者：
Delaney, Christopher;O'Donnell, Lindsay E.;Ramage, Gordon
背景：支持念珠菌属在口腔疾病中作用的证据有限。这种机会性酵母通常被认为是共生的，已被证明在假牙相关疾病中发挥着作用，尽管它是积极的参与者还是无辜的旁观者仍有待确定。本研究旨在了解假牙患者群体中念珠菌属物种与细菌微生物组的作用，探索口腔卫生习惯如何影响假牙微生物学。材料和方法：在复杂的 9 种跨界假牙生物膜模型上进行体外假牙清洁研究，并对残留的细菌和真菌活生物负载进行定量评估。还收集了 131 名患者的患者卫生措施，包括 OHIP、假牙清洁频率、口腔卫生措施和患者人口统计数据。对每位患者的细菌微生物组进行分析，同时对义齿、粘膜和完整牙列的 ITS（真菌）和 16S（细菌）生物负荷进行定量 PCR 评估。 结果：结果显示，体外义齿清洁后，白色念珠菌对治疗没有反应，而细菌生物膜可以重新繁殖 100 倍，但对后续治疗敏感。在患者队列中，口腔卫生不会影响念珠菌或细菌的组成，也不会影响多样性。念珠菌的水平并没有显着影响细菌微生物组，尽管观察到的梯度表明微生物组成随念珠菌负荷而变化，表明界间相互作用而不是口腔卫生效应。事实上，相关性分析能够显示念珠菌属物种和关键属（乳杆菌属、斯卡多菌属、梭杆菌属）之间的显着相关性。结论：总体而言，这项研究表明，假牙微生物组/真菌组对口腔卫生挑战相对有弹性，但念珠菌属物种与关键口腔属存在潜在的相互作用。这些相互作用可能会对塑造社区结构产生影响，并在机会出现时影响从健康到疾病的转变。
Background: Evidence to support the role of Candida species in oral disease is limited. Often considered a commensal, this opportunistic yeast has been shown to play a role in denture related disease, though whether it is an active participant or innocent bystander remains to be determined. This study sought to understand the role of Candida species alongside the bacterial microbiome in a denture patient cohort, exploring how the microbiology of the denture was affected by oral hygiene practices.Materials and methods: In vitro denture cleansing studies were performed on a complex 9-species interkingdom denture biofilm model, with quantitative assessment of retained bacterial and fungal viable bioburdens. Patient hygiene measures were also collected from 131 patients, including OHIP, frequency of denture cleansing, oral hygiene measure and patient demographics. The bacterial microbiome was analysed from each patient, alongside quantitative PCR assessment of ITS (fungal) and 16S (bacterial) bioburden from denture, mucosa and intact dentition.Results: It was shown that following in vitro denture cleansing C. albicans were unresponsive to treatment, whereas bacterial biofilms could repopulate 100-fold, but were susceptible to subsequent treatment. Within the patient cohort, oral hygiene did not impact candidal or bacterial composition, nor diversity. The levels of Candida did not significantly influence the bacterial microbiome, though an observed gradient was suggestive of a microbial composition change in response to Candida load, indicating interkingdom interaction rather than an oral hygiene effect. Indeed, correlation analysis was able to show significant correlations between Candida species and key genera (Lactobacillus, Scardovia, Fusobacterium).Conclusions: Overall, this study has shown that the denture microbiome/mycobiome is relatively resilient to oral hygiene challenges, but that Candida species have potential interactions with key oral genera. These interactions may have a bearing on shaping community structure and a shift from health to disease when the opportunity arises.