Cell traces - Footprints of individual cells during locomotion and adhesion
Cell traces - Footprints of individual cells during locomotion and adhesion
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DOI：
10.1515/bchm.1998.379.8-9.1161
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发表时间：
1998-08-01
影响因子：
3.7
通讯作者：
Hagedorn, R
中科院分区：
文献类型：
--
作者：
Fuhr, G;Richter, E;Hagedorn, R
动物细胞在粘附和迁移过程中会在玻璃或硅表面释放微量物质，这是细胞迁移过程中普遍存在的现象，本文介绍了这类物质的研究情况。这些痕迹可以通过不同的显微镜技术（例如TIRF，SEM，CLSM，AFM，SEM）可视化。显示并讨论了不同细胞系（NIH 3T3和L929小鼠成纤维细胞、小鼠巨噬细胞、小鼠肉瘤细胞和人骨肉瘤细胞）的典型细胞迹线，有组织良好的结构，如不同的线性和结节状元素以及斑块。迹线可以从细胞延伸到几百微米，但是线性元件的尺寸在亚微米范围内。细胞迹线与焦点接触不相同，但是可以包括它们。提出了基本元件的第一分类，其允许与供体细胞相比估计总体积和表面，高阶结构进行了讨论，并给出了第一次深入了解小鼠成纤维细胞产生的痕迹的蛋白质组成。我们的观察结果以及细胞粘附文献表明，释放材料的量、其程度以及化学和结构特性取决于细胞类型和生理学以及其他外部影响。细胞迹线结合供体细胞的粘附模式，可以提供有关单个细胞的活动和生理状态、细胞运动机制和供体细胞膜的分子组成的信息。这些迹线可能用作被动电表征和生物技术应用的亚微米元件。
Animal cells release traces of material onto glass or silicon surfaces during adhesion and migration, This little studied phenomenon is a widespread and normal concomitant of cell migration, The paper introduces the study of such material. The traces can be visualised by different microscopic techniques (e.g. TIRF, IRM, CLSM, AFM, SEM). Cell traces typical for different cell lines (NIH 3T3 and L929 mouse fibroblasts, mouse macrophages, mouse sarcoma cells and human osteosarcoma cells)are shown and discussed, There are well organised structures such as different linear and nodular elements as well as patches. Traces can extend up to some hundred micrometers from the cell, but the dimensions of the linear elements are in the submicron range, Cell traces are not identical with focal contacts but can include them, A first classification of basic elements is proposed, It allows an estimation of the total volume and surface in comparison to the donor cell, Higher order structures are discussed and a first insight into the protein composition of traces produced by mouse fibroblasts is given. Our observations, together with the cell adhesion literature suggest that the amount of released material, its extent and chemical and structural properties depend on cell type and physiology as well as other external influences. Cell traces in combination with the adhesion pattern of the donor cell should give information about the activity and physiological status of individual cells, the mechanisms of cell locomotion and the molecular composition of the donor cell membrane, The traces might possibly be used as submicron elements for passive electric characterisation and biotechnological applications.