Patterned cell adhesion by self-assembled structures for use with a CMOS cell-based biosensor
Patterned cell adhesion by self-assembled structures for use with a CMOS cell-based biosensor
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DOI：
10.1016/j.bios.2006.06.031
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发表时间：
2007-02-15
影响因子：
12.6
通讯作者：
Hierlemann, A.
中科院分区：
文献类型：
--
作者：
Franks, W.;Tosatti, S.;Hierlemann, A.
提出了一种基于化学表面改性的图案化细胞粘附策略。为了将细胞粘附限制在特定位置，已经开发了用于高对比度蛋白质吸附的工程表面，从而实现细胞附着。表面功能化基于选择性分子组装图案化（SMAP）。胺封端的自组装单层用于限定细胞粘附的区域。蛋白质排斥性接枝共聚物，聚（L-赖氨酸）-接枝-聚（乙二醇）（PLL-g-PEG），用于使周围的二氧化硅抵抗蛋白质吸附。X射线光电子能谱，扫描椭圆偏振和荧光显微镜技术被用来监测图案化过程的各个步骤。使用这些层的成功引导生长在体外用原代新生大鼠心肌细胞（长达4天）和用HL-1心肌细胞系（长达7天）证明。所提出的方法的优点是，高分辨率的工程表面可以实现使用一个简单的，具有成本效益的，浸渍和冲洗过程。该技术已被开发用于基于CMOS细胞的生物传感器上，该生物传感器包括微电极阵列以细胞外记录心肌细胞的电活动。(c)2006 Elsevier B. V.保留所有权利。
A strategy for patterned cell adhesion based on chemical surface modification is presented. To confine cell adhesion to specific locations, an engineered surface for high-contrast protein adsorption and, hence, cell attachment has been developed. Surface functionalization is based on selective molecular-assembly patterning (SMAP). An amine-terminated self-assembled monolayer is used to define areas of cell adhesion. A protein-repellent grafted copolymer, poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG), is used to render the surrounding silicon dioxide resistant to protein adsorption. X-ray photoelectron spectroscopy, scanning ellipsometry and fluorescence microscopy techniques were used to monitor the individual steps of the patterning process. Successful guided growth using these layers is demonstrated with primary neonatal rat cardiomyocytes, up to 4 days in vitro, and with the HL-1 cardiomyocyte cell line, up to 7 days in vitro. The advantage of the presented method is that high-resolution engineered surfaces can be realized using a simple, cost-effective, dip-and-rinse process. The technique has been developed for application on a CMOS cell-based biosensor, which comprises an array of microelectrodes to extracellularly record electrical activity from cardiomyocytes. (c) 2006 Elsevier B.V. All rights reserved.