Human recombinant interleukin 4 induces Fc epsilon R2/CD23 on normal human monocytes.
Human recombinant interleukin 4 induces Fc epsilon R2/CD23 on normal human monocytes.
复制标题
人重组白细胞介素 4 在正常人单核细胞上诱导 Fc epsilon R2/CD23。
DOI：
10.1084/jem.167.4.1406
复制
发表时间：
1988-04-01
影响因子：
15.3
通讯作者：
Leung, D Y
中科院分区：
文献类型：
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作者：
Vercelli, D;Jabara, H H;Lee, B W;Woodland, N;Geha, R S;Leung, D Y
rIL-4（B细胞刺激因子1）诱导正常人单核细胞（Mo）上Fc受体2/CD 23的表达。通过使用抗CD 23 mAb和可溶性IgE的流式细胞术，以及通过使用来自125 I-乳过氧化物酶标记Mo的45 kD条带的CD 23特异性mAb或IgE的免疫沉淀，可检测Fc CD 2 R2/CD 23诱导。在与rIL-4孵育24小时后，Fc CD 2 R2/CD 23完全表达，并且在加入IL-4后72小时仍可检测到。该效应是特异性的，因为测试的其他rIL（IL-1、IL-2、IL-3、IL-5、B细胞刺激因子2、粒细胞-巨噬细胞集落刺激因子和IFN-γ）均不能单独或以各种组合诱导FC miR 2/CD 23。IL-4与其他IL之间无协同作用。IFN-γ不能抑制IL-4诱导的Mo上Fc γ R2/CD 23的表达，当与IL-4一起加入培养物中时，也不能抑制36小时前加入时。
rIL-4 (B cell stimulatory factor 1) induces the expression of Fc epsilon R2/CD23 on normal human monocytes (Mo). Fc epsilon R2/CD23 induction was detectable both by flow cytometry using anti-CD23 mAbs as well as soluble IgE, and by the immunoprecipitation with CD23-specific mAb or IgE of a 45-kD band from 125I-lactoperoxidase-labeled Mo. Fc epsilon R2/CD23 was fully expressed after a 24-h incubation with rIL-4, and was still detectable after 72 h from the addition of IL-4. This effect was specific, because none of the other rILs tested (IL-1, IL-2, IL-3, IL-5, B cell stimulatory factor 2, granulocyte-macrophage colony stimulating factor, and IFN-gamma) could induce FC epsilon R2/CD23, either alone or in various combinations. No synergism was observed between IL-4 and other ILs. IFN-gamma was not able to inhibit the IL-4- induced expression of Fc epsilon R2/CD23 on Mo, neither when added to the culture together with IL-4, nor when added 36 h earlier.