Expression of a Pennisetum glaucum gene DREB2A confers enhanced heat, drought and salinity tolerance in transgenic Arabidopsis
Expression of a Pennisetum glaucum gene DREB2A confers enhanced heat, drought and salinity tolerance in transgenic Arabidopsis
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DOI：
10.1007/s11033-022-07527-6
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发表时间：
2022-06-06
影响因子：
2.8
通讯作者：
Padaria, Jasdeep Chatrath
中科院分区：
文献类型：
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作者：
Meena, Rajendra Prasad;Ghosh, Gourab;Padaria, Jasdeep Chatrath
珍珠粟（Pennisetumglaucum）是一种重要的粮食作物，其生长和产量不受干旱、高温和低温等非生物胁迫的影响。DREB转录因子是植物中最大的一类转录因子，在植物逆境应答和信号转导中发挥着重要作用。方法和结果在本研究中，PgDREB 2A基因编码的DREB转录因子在珍珠粟在拟南芥中的功能进行了表征。DREB 2A蛋白含有一个保守的结构域，它与乙烯反应元件结合因子结合。Southern杂交结果表明，获得了3个高表达PgDREB 2A基因的T-1转基因株系。实时荧光定量聚合酶链反应结果表明，干旱条件下PgDREB 2A基因可以被诱导表达。与对照相比，PgDREB 2A过表达转基因拟南芥在高浓度甘露醇、NaCl、阿坝、高温和低温胁迫下，种子萌发率和根系生长速率均有所提高。此外，PgDREB 2A转基因株系在复水后表现出增强的耐久性，并且对干旱和盐胁迫的耐受性随着脯氨酸的增加和MDA积累的减少以及水分损失的减少而增强。结论PgDREB 2A作为一种转录因子，可以提高作物对各种非生物胁迫的耐受性，可用于培育耐非生物胁迫的作物。
Background Pearl millet (Pennisetum glaucum) is an essential cereal crop, whose growth and yield are not impacted by abiotic stresses, such as drought, heat, and cold. The DREB transcription factors (TF) are some of the largest groups of TFs in plants and play varied roles in plant stress response and signal transduction. Methods and results In the present study, PgDREB2A gene encoding a DREB transcription factor in pearl millet was functionally characterized in Arabidopsis. DREB2A proteins contain a conserved domain that binds toethylene responsive element binding factors. Three different T-1 transgenic lines overexpressing PgDREB2A gene were identified by Southern blot. Quantitative real-time polymerase chain reaction exhibited that PgDREB2A could be induced under drought conditions. As compared with the control, PgDREB2A overexpressing transgenic Arabidopsis showed increased rate of seed germination and root growth in transgenic lines under higher concentrations of mannitol, NaCl, ABA, heat and cold stress. Additionally, PgDREB2A transgenic lines showed enhanced durability after rehydration and tolerance to drought and salt stress was augmented with increased proline and reduced MDA build-up and diminishing water loss. Conclusions Results from this study suggested that PgDREB2A as a transcription factor may improve endurance to various abiotic stresses and can be employed for developing crops tolerant to abiotic stresses.