Experimental paratuberculosis (Johne's disease)--studies on biochemical parameters in cattle.
Experimental paratuberculosis (Johne's disease)--studies on biochemical parameters in cattle.
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实验性副结核病（约翰氏病）——牛生化参数的研究。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
G. Nagy
中科院分区：
文献类型：
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作者：
M. Szilágyi;B. Körmendy;A. Súri;S. Tuboly;G. Nagy
10只自然感染副结核分枝杆菌的雄性荷斯坦-弗里西亚犊牛在平均17天后再次被口服感染。在感染后160天至400天期间，每月对以下活动进行测量：总蛋白（TPR）、白蛋白（ALB）、胆固醇（CHOL）、甘油三酯（TRIG）、锌和铜浓度以及山梨醇脱氢酶、乳酸脱氢酶（LDH）、α -羟基丁酸脱氢酶（α - hbdh）、γ -谷氨酰转移酶、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、肌酸激酶（CK）、碱性磷酸酶和果糖-1,6-二磷酸醛缩酶（ALD）。到第400天，TPR、ALB、TRIG和CHOL降低，并伴有消化和吸收障碍。CK、ALD、LDH、α - hbdh、AST和ALT活性升高主要表明骨骼肌和/或肝脏受损。血清CK和ALD活性以及TRIG和TPR浓度可能有助于副结核的特异性诊断，特别是在疾病的晚期。
Ten male Holstein-Friesian calves naturally infected by Mycobacterium paratuberculosis were experimentally re-infected orally at an average of 17 days. Monthly measurements were conduced of the following activities, in the period between post infection days 160 and 400: total protein (TPR), albumin (ALB), cholesterol (CHOL), triglycerides (TRIG), Zn and Cu concentrations as well as sorbitol dehydrogenase, lactate dehydrogenase (LDH), alpha-hydroxybutyrate dehydrogenase (alpha-HBDH), gamma-glutamyltransferase, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), alkaline phosphatase and fructose-1,6-diphosphate aldolase (ALD). TPR, ALB, TRIG, and CHOL were reduced by day 400, in conjunction with disorders of digestion and absorption. Increased activities of CK, ALD, LDH, alpha-HBDH, AST and ALT primarily indicated damage to skeletal muscle and/or liver. Serum CK and ALD activities as well as TRIG and TPR concentrations may serve as aids to specific diagnosis of paratuberculosis, particularly in the advanced stage of the disease.