Caenorhabditis elegans NSE3 homolog (MAGE-1) is involved in genome stability and acts in inter-sister recombination during meiosis
Caenorhabditis elegans NSE3 homolog (MAGE-1) is involved in genome stability and acts in inter-sister recombination during meiosis
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DOI：
10.1093/genetics/iyad149
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发表时间：
2023-10-04
期刊：
影响因子：
3.3
通讯作者：
Wang，Bin
中科院分区：
文献类型：
--
作者：
Odiba，Arome Solomon;Liao，Guiyan;Wang，Bin
黑色素瘤抗原（MAGE）基因编码一个具有共同MAGE同源结构域的蛋白家族。这些基因在真核生物中是保守的，并且与多种细胞和发育过程有关，包括泛素化和癌症的肿瘤发生。目前关于MAGE家族蛋白的知识主要来自酵母和人类细胞系的分析，它们在动物机体水平上的功能尚未报道。秀丽隐杆线虫只编码1个已知的MAGE基因成员MAGE -1（酵母中的nse3），形成SMC-5/6复合体的一部分。在这里，我们描述了mage-1/nse-3在线虫有丝分裂和减数分裂中的作用。mage-1/nse-3在减数分裂重组过程中参与姐妹间重组修复，并在多种dna损伤剂治疗后保持染色体完整性。MAGE-1直接与NSE-1和NSE-4相互作用。与smc-5、smc-6和nse-4突变体相比，mage-1/nse-3突变体的NSE-1::GFP水平降低，残余的NSE-1::GFP部分是核，大部分是细胞质。我们的数据表明，MAGE-1对于NSE-1的稳定性和SMC-5/6复合物的正常功能至关重要。
Melanoma antigen (MAGE) genes encode for a family of proteins that share a common MAGE homology domain. These genes are conserved in eukaryotes and have been linked to a variety of cellular and developmental processes including ubiquitination and oncogenesis in cancer. Current knowledge on the MAGE family of proteins mainly comes from the analysis of yeast and human cell lines, and their functions have not been reported at an organismal level in animals.Caenorhabditis elegansonly encodes 1 known MAGE gene member,mage-1(NSE3in yeast), forming part of the SMC-5/6 complex. Here, we characterize the role ofmage-1/nse-3in mitosis and meiosis inC. elegans.mage-1/nse-3has a role in inter-sister recombination repair during meiotic recombination and for preserving chromosomal integrity upon treatment with a variety of DNA-damaging agents. MAGE-1 directly interacts with NSE-1 and NSE-4. In contrast tosmc-5,smc-6,andnse-4mutants which cause the loss of NSE-1 nuclear localization and strong cytoplasmic accumulation,mage-1/nse-3mutants have a reduced level of NSE-1::GFP, remnant NSE-1::GFP being partially nuclear but largely cytoplasmic. Our data suggest that MAGE-1 is essential for NSE-1 stability and the proper functioning of the SMC-5/6 complex.