ISSR ANALYSIS OF THE HYBRID DESCENDANTS OF TEA CAMELLIAS
ISSR ANALYSIS OF THE HYBRID DESCENDANTS OF TEA CAMELLIAS
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发表时间：
2006
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通讯作者：
Hou Yu-jia
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作者：
Hou Yu-jia
简单序列重复序列(ISSR)是在微卫星或简单序列重复序列(SSR)的基础上发展起来的一种有效的分子标记。本研究利用19个ISSR引物,利用ISSR技术对9个茶树亲本及其杂交后代进行遗传分析鉴定。茶树基因型的GS值在0.594~0.797之间,其中树勇之间GS最大。 307和蜀永906遗传相似度最高，遗传距离最小。云南大叶茶和四川中小叶种之间的GS最小，遗传相似度最低，遗传差异最大。聚类分析将9个基因型分为3大类群，云南大叶茶和蜀永1号为一组，原始型列二茶为一组，其余为第三类，提示：结果表明ISSR标记可作为一种有效的分子技术用于“亲缘关系追踪”、子代定向选择和茶树杂交育种研究。
Inter-simple sequence repeat(ISSR) is a kind of effective molecular marker developed on the basis of microsatellite,or simple sequence repeat(SSR).In the present study,19 ISSR primers were utilized to analyze and identify the heredity in 9 parent materials of tea trees and their hybrid descendants with ISSR technology.The GS values of the tea genotypes ranged from 0.594 to 0.797.The GS was the greatest between Shuyong 307 and Shuyong 906 and they had the highest genetic similarity and the smallest genetic distance.The GS between Yunnan-Dayecha and Sichuan Zhongxiaoyezhong was the smallest and they had the lowest genetic similarity and the greatest genetic difference.Cluster analysis classified the 9 genotypes into 3 major groups.Yunnan-Dayecha and Shuyong 1 fell into one group.Liercha,a primitive form,constituted one group.The rest formed the third group,suggesting that they are close to one another genetically.The results suggest t hat ISSR markers can be used as an effective molecular technique for "genetic relationship following",directional selection of the filial generation and the study of tea cross-breeding.