Neuropeptide Y-induced phase shifts of PER2::LUC rhythms are mediated by long-term suppression of neuronal excitability in a phase-specific manner.
Neuropeptide Y-induced phase shifts of PER2::LUC rhythms are mediated by long-term suppression of neuronal excitability in a phase-specific manner.
复制标题
DOI：
10.3109/07420528.2011.649382
复制
发表时间：
2012-03
影响因子：
2.8
通讯作者：
Gamble KL
中科院分区：
文献类型：
--
作者：
Besing RC;Hablitz LM;Paul JR;Johnson RL;Prosser RA;Gamble KL
Endogenous circadian rhythms are entrained to the 24-h light/dark cycle by both light and nonphotic stimuli. During the day, nonphotic stimuli, such as novel-wheel induced exercise, produce large phase advances. Neuropeptide Y (NPY) release from the thalamus onto suprachiasmatic nucleus (SCN) neurons at least partially mediates this nonphotic signal. We examined the hypothesis that NPY-induced phase advances are accompanied by suppression of PER2 and are mediated by long-term depression of neuronal excitability in a phase-specific manner. First, we found that NPY-induced phase advances in PER2::LUC SCN cultures are largest when NPY (2.35 µM) is given in the early part of the day (circadian time [CT] 0–6). In addition, PER2::LUC levels in NPY-treated (compared to vehicle-treated) samples were suppressed beginning 6–7 h after treatment. Similar NPY application to organotypic Per1::GFP SCN cultures resulted in long-term suppression of spike rate of GFP+ cells when slices were treated with NPY during the early or middle of the day (zeitgeber time [ZT] 2 or 6), but not during the late day (ZT 10). Furthermore, 1-h bath application of NPY to acute SCN brain slices decreased general neuronal activity measured through extracellular recordings. Finally, NPY-induced phase advances of PER2::LUC rhythms were blocked by latent depolarization with 34.5 mM [K+] 3 h after NPY application. These results suggest that NPY-induced phase advances may be mediated by long-term depression of neuronal excitability. This model is consistent with findings in other brain regions that NPY-induced persistent hyperpolarization underlies mechanisms of energy homeostasis, anxiety-related behavior, and thalamocortical synchronous firing.
登录
查看更多内容
影响因子：
--
作者：
ALBERS, HE;OTTENWELLER, JE;ANDERSON, ER
通讯作者：
ANDERSON, ER
影响因子：
5.3
作者：
Han, MH;Bolanos, CA;Nestler, EJ
通讯作者：
Nestler, EJ
影响因子：
2.5
作者：
Fetissov, SO;Byrne, LC;Hökfelt, T
通讯作者：
Hökfelt, T
影响因子：
1.7
作者：
Golombek, DA;Biello, SM;Harrington, ME
通讯作者：
Harrington, ME
影响因子：
5.3
作者：
Gamble, Karen L.;Allen, Gregg C.;McMahon, Douglas G.
通讯作者：
McMahon, Douglas G.