A wirelessly controlled implantable LED system for deep brain optogenetic stimulation.
A wirelessly controlled implantable LED system for deep brain optogenetic stimulation.
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DOI：
10.3389/fnint.2015.00008
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发表时间：
2015
影响因子：
3.5
通讯作者：
Yin HH
中科院分区：
文献类型：
--
作者：
Rossi MA;Go V;Murphy T;Fu Q;Morizio J;Yin HH
近年来，光遗传学已迅速成为神经科学中的一项重要技术。它的时间和空间特异性，结合操纵神经元活动的功效，在研究清醒行为动物的行为方面特别有用。然而，传统的光遗传学需要使用激光和光纤，这可能会对行为产生相当大的限制。在这里，我们结合了无线控制接口和小型可植入发光二极管（LED），允许灵活和精确地放置光源来照亮任何大脑区域。我们在体内测试了这种无线LED系统，在表达D1样多巴胺受体的纹状体黑质神经元中表达通道视紫红质-2的转基因小鼠中。在所有测试的小鼠中，我们都能够可靠地引发运动。由高功率刺激诱导的抽搐的频率与刺激的频率成比例。在较低的功率下，观察到反向转向。此外，植入的LED在手术后50天内仍然有效，证明了光源的长期稳定性。我们的研究结果表明，无线LED系统可用于长期操纵行为小鼠的神经活动，而不会阻碍自然运动。
In recent years optogenetics has rapidly become an essential technique in neuroscience. Its temporal and spatial specificity, combined with efficacy in manipulating neuronal activity, are especially useful in studying the behavior of awake behaving animals. Conventional optogenetics, however, requires the use of lasers and optic fibers, which can place considerable restrictions on behavior. Here we combined a wirelessly controlled interface and small implantable light-emitting diode (LED) that allows flexible and precise placement of light source to illuminate any brain area. We tested this wireless LED system in vivo, in transgenic mice expressing channelrhodopsin-2 in striatonigral neurons expressing D1-like dopamine receptors. In all mice tested, we were able to elicit movements reliably. The frequency of twitches induced by high power stimulation is proportional to the frequency of stimulation. At lower power, contraversive turning was observed. Moreover, the implanted LED remains effective over 50 days after surgery, demonstrating the long-term stability of the light source. Our results show that the wireless LED system can be used to manipulate neural activity chronically in behaving mice without impeding natural movements.