Sequence-specific control of azobenzene assemblies by molecular recognition of DNA
Sequence-specific control of azobenzene assemblies by molecular recognition of DNA
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DOI：
10.1021/la702720g
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发表时间：
2008-03-18
期刊：
影响因子：
3.9
通讯作者：
Ijiro, Kuniharu
中科院分区：
文献类型：
--
作者：
Haruta, Osamu;Matsuo, Yasutaka;Ijiro, Kuniharu
我们研究了由分子疏水部分的偶氮苯和亲水部分的腺嘌呤组成的两亲物 AzoAde 与具有均质碱基（dA(30)、dT(30)、dG(30) 和 dC(30)）的寡核苷酸在空气-水界面处的分子识别。基于双链体中 DNA 互补碱基配对，使用 pi-A 等温线、UV-vis RAS、FT-IR RAS 和 XPS 测量检查 AzoAde 在模板化 dT(30) 上的有序排列。尽管AzoAde和错配寡核苷酸（dA（30）、dG（30）和dC（30））之间几乎没有相互作用，但在dT（30）上制备的AzoAde与dT（30）相互作用，随后在空气-水界面形成J型组装体。 LB 膜的 AFM 观察揭示了 dT(30) 亚相上 J 形成的 AzoAde 单层的纳米结构以及其他寡核苷酸亚相上 H 形成的单层的结构域结构。因此，dT(30)具有作为在空气-水界面组装AzoAde的模板的潜在应用。
We investigated the molecular recognition between the amphiphile AzoAde, which is composed of azobenzene in the hydrophobic and adenine in the hydrophilic portion of the molecule, and oligonucleotides having a homogeneous base (dA(30), dT(30), dG(30), and dC(30)) at the air-water interface. On the basis of the complementary base-pairing of DNA in the duplex, orderly arrangement of AzoAde on templated dT(30) was examined using pi-A isotherm, UV-vis RAS, FT-IR RAS, and XPS measurements. Although there was little interaction between AzoAde and mismatched oligonucleotides (dA(30), dG(30) and dC(30)), AzoAde prepared on a dT(30) interacted with dT(30), subsequently forming a J-form assembly at the air-water interface. AFM observation of the LB films revealed the nanostructure of the J-formed AzoAde monolayer on the dT(30) subphase as well as the domain structures of the H-formed monolayers on the other oligonucleotide subphases. Therefore, dT(30) has a potential application as a template for assembling AzoAde at the air-water interface.