Succinylation proteomic analysis identified differentially expressed succinylation sites affecting porcine muscle fiber type function.
Succinylation proteomic analysis identified differentially expressed succinylation sites affecting porcine muscle fiber type function.
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DOI：
10.1016/j.fochx.2023.100962
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发表时间：
2023-12-30
期刊：
影响因子：
6.1
通讯作者：
Bojiang Li
中科院分区：
文献类型：
--
作者：
Xiaofan Tan;Kaiqing Liu;Yu He;Zhiwei Yan;Jing Chen;Ruixue Zhao;Xin Sui;Junpeng Zhang;David M. Irwin;Shuyi Zhang;Bojiang Li
我们在BF和SOL样品之间进行了蛋白质琥珀酸化图谱的研究。共鉴定出294个差异表达的琥珀酸化位点(DESS)。含有DESS的蛋白质参与了糖酵解、三羧酸循环(TCA)。构建了一个基于含有DESS的蛋白质的PPI分析。肌肉纤维类型是影响猪肉品质的主要因素，然而，翻译后蛋白修饰，特别是琥珀酸化在肌肉纤维类型调控中的作用还不完全清楚。在这里，我们研究了快型股二头肌(BF)和慢型比目鱼肌(SOL)的蛋白质琥珀酸化图谱。从这些样本中鉴定出4,221个琥珀酸化位点，其中294个位点存在差异表达。基因本体论(GO)和京都基因和基因组百科全书(KEGG)的浓缩分析表明，这些琥珀酸化的蛋白质主要参与糖酵解、三羧酸循环和脂肪酸代谢。免疫共沉淀法验证了CRAT和RAB10蛋白的琥珀酸化修饰。蛋白质-蛋白质相互作用(PPI)网络分析揭示了这些琥珀酸化蛋白质之间的相互作用，这些蛋白质调控着猪肌纤维类型的转换。本研究为蛋白质琥珀酸化在猪肌纤维类型转化和肉质调控中的分子作用提供了新的视角。
We performed protein succinylation profiles between BF and SOL samples. A total of 294 differentially expressed succinylation sites (DESSs) were identified. The proteins containing DESSs were involved in the glycolysis, tricarboxylic acid cycle (TCA). A PPI analysis was constructed based on proteins containing DESSs. Muscle fiber type is a major factor in pork meat quality, however, the role of post-translational protein modifications, especially succinylation, in the regulation of muscle fiber type is not fully understood. Here we performed protein succinylation profiles of fast-type biceps femoris (BF) and slow-type soleus (SOL) muscles. A total of 4,221 succinylation sites were identified from these samples, of which 294 sites were differentially expressed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that these succinylated proteins were mainly involved in glycolysis, tricarboxylic acid cycle, and fatty acid metabolism. Succinylation modification of the CRAT and RAB10 proteins was verified by co-immunoprecipitation. Protein-protein interaction (PPI) network analysis unveiled the interactions of these succinylated proteins that regulate pig myofiber type conversion. This investigation offers fresh perspectives into the molecular roles of protein succinylation in the regulation of pig myofiber type transformation and meat quality.
影响因子：
5
作者：
He, Yu;Tan, Xiaofan;Li, Hongqiang;Yan, Zhiwei;Chen, Jing;Zhao, Ruixue;Irwin, David M.;Wu, Wangjun;Zhang, Shuyi;Li, Bojiang
通讯作者：
Li, Bojiang