Molecular analysis of Dehalococcoides 16S ribosomal DNA from chloroethene-contaminated sites throughout north America and Europe
Molecular analysis of Dehalococcoides 16S ribosomal DNA from chloroethene-contaminated sites throughout north America and Europe
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DOI：
10.1128/aem.68.2.485-495.2002
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发表时间：
2002-02-01
影响因子：
4.4
通讯作者：
Ebersole, RC
中科院分区：
文献类型：
--
作者：
Hendrickson, ER;Payne, JA;Ebersole, RC
研究了脱卤球菌群生物的环境分布及其与氯乙烯污染地点的关联。通过使用为检测 Dehalococcoides 16S rRNA 基因 (rDNA) 序列而开发的 PCR 检测方法，对来自分布在北美和欧洲的 24 个氯乙烯脱氯站点的样品进行了检测，以确定 Dehalococcoides 群体成员的存在。通过序列分析，在 21 个位点检测到了 Dehalococcoides 群成员的序列。在这些地点，氯乙烯完全脱氯为乙烯。在氯乙烯发生部分脱氯的三个地点的样品中未检测到脱卤球菌序列，其中脱氯似乎在 1,2-顺式二氯乙烯处停止。 16S rDNA 扩增子的系统发育分析证实 Dehalococcoides 序列形成了独特的 16S rDNA 群。根据 Dehalococcoides 16S rDNA 序列可变区 2 和 6 中的特定碱基取代模式，将这些 16S rDNA 序列分为三个亚组。分析还表明，特定的碱基替换模式是特征模式。特定的碱基替换在系统发育上区分了三个序列亚组。这些结果表明，脱卤球菌类的成员在自然界中分布广泛，可以在各种地质构造和不同的气候带中找到。此外，这些生物体与氯乙烯完全脱氯的关联表明它们是工程生物修复的有希望的候选者，并且可能是氯乙烯自然衰减的重要贡献者。
The environmental distribution of Dehalococcoides group organisms and their association with chloroethene-contaminated sites were examined. Samples from 24 chloroethene-dechlorinating sites scattered throughout North America and Europe were tested for the presence of members of the Dehalococcoides group by using a PCR assay developed to detect Dehalococcoides 16S rRNA gene (rDNA) sequences. Sequences identified by sequence analysis as sequences of members of the Dehalococcoides group were detected at 21 sites. Full dechlorination of chloroethenes to ethene occurred at these sites. Dehalococcoides sequences were not detected in samples from three sites at which partial dechlorination of chloroethenes occurred, where dechlorination appeared to stop at 1,2-cis-dichloroethene. Phylogenetic analysis of the 16S rDNA amplicons confirmed that Dehalococcoides sequences formed a unique 16S rDNA group. These 16S rDNA sequences were divided into three subgroups based on specific base substitution patterns in variable regions 2 and 6 of the Dehalococcoides 16S rDNA sequence. Analyses also demonstrated that specific base substitution patterns were signature patterns. The specific base substitutions distinguished the three sequence subgroups phylogenetically. These results demonstrated that members of the Dehalococcoides group are widely distributed in nature and can be found in a variety of geological formations and in different climatic zones. Furthermore, the association of these organisms with full dechlorination of chloroethenes suggests that they are promising candidates for engineered bioremediation and may be important contributors to natural attenuation of chloroethenes.