Age-associated changes in glutathione peroxidase and oxidized protein in the erythrocytes of senescence-accelerated mice.
Age-associated changes in glutathione peroxidase and oxidized protein in the erythrocytes of senescence-accelerated mice.
复制标题
衰老加速小鼠红细胞中谷胱甘肽过氧化物酶和氧化蛋白与年龄相关的变化。
DOI：
10.4327/jsnfs.43.121
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
K. Yasumoto
中科院分区：
文献类型：
--
作者：
P. He;K. Yasumoto
从衰老加速小鼠（包括易感菌株（SAM-P/1）和耐药菌株（SAM-R/1））获得的新鲜红细胞被分成 4 种不同的细胞密度，即e.细胞年龄，密度梯度离心的分数。丙酮酸激酶和己糖激酶被认为是红细胞细胞年龄的标志酶，在两种小鼠品系中，丙酮酸激酶和己糖激酶的比活性随着红细胞密度的增加而降低，并且在某些细胞组分中，SAM-P/1 比 SAM-R/1 更高。两种菌株中谷胱甘肽还原酶的比活性随着细胞密度的增加而降低。两种品系中谷胱甘肽过氧化物酶的比活性也随着细胞密度的增加而降低，但来自 SAM-P/1 的大多数级分低于来自 SAM-R/1 的相应级分。通过与 2, 4-二硝基苯肼的反应性评估的氧化蛋白水平随着两种小鼠品系中细胞密度的增加而增加，并且来自 SAM-P/1 的所有级分均高于来自 SAM-R/1 的氧化蛋白水平。这些结果被解释为表明 SAM-P/1 红细胞的衰老过程加速，并且这种加速部分与细胞氧化防御机制（包括谷胱甘肽过氧化物酶）的缺陷有关。
Fresh erythrocytes obtained from senescence-accelerated mice, both prone (SAM-P/1) and resistant (SAM-R/1) strains, were separated into 4 different cell density, i. e. cell age, fractions by densitygradient centrifugation. The specific activities of pyruvate kinase and hexokinase, which are known to be marker enzymes of cell age for erythrocytes, decreased with increased erythrocyte cell density in both strains of mouse, and in some cell fractions were higher in SAM-P/1 than in SAM-R/1. The specific activity of glutathione reductase decreased with increased cell density in both strains. The specific activity of glutathione peroxidase also decreased with increased cell density in both strains, but was lower in most fractions from SAM-P/1 than in the corresponding fractions from SAM-R/1.The level of oxidized protein assessed by reactivity with 2, 4-dinitrophenylhydrazine increased with the increase in cell density in both strains of mouse and was higher in all the fractions from SAM-P/1 than in those from SAM-R/1. These results are interpreted to indicate that the aging process is accelerated in the erythrocytes of SAM-P/1, and that this acceleration is partly associated with some defect in the cellular oxidant defense mechanism, including glutathione peroxidase.