Dynamic motions and architectural changes in DNA supramolecular aggregates visualized via transmission electron microscopy without liquid cells.
Dynamic motions and architectural changes in DNA supramolecular aggregates visualized via transmission electron microscopy without liquid cells.
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DOI：
10.1039/d1nr04133a
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发表时间：
2021-09
期刊：
影响因子：
6.7
通讯作者：
Zhuoyang Lu;Xiangyang Liu;M. He;J. Long;Jiankang Liu
中科院分区：
文献类型：
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作者：
Zhuoyang Lu;Xiangyang Liu;M. He;J. Long;Jiankang Liu
在过去的十年中，液相透射电子显微镜（TEM）的突破，使原位可视化的纳米结构在液体介质中的运动动力学与前所未有的细节。然而，它仍然是一个重大的挑战，以执行液相TEM由于复杂的制备过程中的液体细胞，以保持液体蒸发在超真空条件下的TEM柱。在本研究中，非挥发性和显着的溶剂化性能的离子液体（ILs）被利用到图像的DNA超分子聚集体和Au纳米粒子（NP）聚集体的动态过程，包括布朗运动，单个纳米物体之间的相互作用和结构的变化在纳米分辨率。DNA超分子聚集体和Au NP聚集体的运动行为存在显著差异。此外，还研究了动态运动的温度和剂量依赖性。研究结果提供了深入了解的DNA超分子聚集体和Au NP聚集体在离子液体中的动力学，并提出了一个容易获得的方法来探测生物大分子和其他软物质聚集体与各种离子液体在纳米尺度上的动力学过程。
In the last decade, breakthroughs in liquid-phase transmission electron microscopy (TEM) have enabled in situ visualization of the motion dynamics of nanostructures in liquid media with unprecedented detail. However, it remains a significant challenge to perform liquid-phase TEM due to the intricate preparation procedure of liquid cells to keep liquid from evaporating under ultrahigh vacuum conditions in TEM columns. In the present study, the nonvolatility and remarkable solvation property of ionic liquids (ILs) is exploited to image the dynamic processes of DNA supramolecular aggregates and Au nanoparticle (NP) aggregates encompassing Brownian motions, interactions among individual nanoobjects and changes in architecture at nanometer resolution. Significant differences in motion behaviors are observed between DNA supramolecular aggregates and Au NP aggregates. Moreover, the temperature and dose dependence of dynamic motions are also investigated. The findings provide insights into the dynamics of DNA supramolecular aggregates and Au NP aggregates in ILs and present an easily accessible approach for probing the dynamic processes of biomacromolecular and other soft matter aggregates with various kinds of ILs at the nanoscale level.