Development and biologic properties of malignant cell sublines and clones of a spontaneously metastasizing rat mammary adenocarcinoma.
Development and biologic properties of malignant cell sublines and clones of a spontaneously metastasizing rat mammary adenocarcinoma.
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DOI：
10.1093/jnci/68.3.507
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发表时间：
1982-03
期刊：
影响因子：
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通讯作者：
A. Neri;D. Welch;T. Kawaguchi;G. Nicolson
中科院分区：
文献类型：
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作者：
A. Neri;D. Welch;T. Kawaguchi;G. Nicolson
利用大鼠13762乳腺癌肿瘤，建立了自发性乳腺肿瘤转移的动物模型。皮下植入雌性Fischer 344大鼠乳腺脂肪垫的亲本肿瘤以低频率转移至淋巴结和肺，未转移至其他器官。卵巢切除术并没有改变亲代 13762 肿瘤的转移特性。细胞系适应来自淋巴结或肺转移的组织培养，并在自发转移测定中与亲代肿瘤移植物进行比较。只有从转移瘤建立的细胞系在皮下植入后23天内自发转移，这表明来自转移瘤的肿瘤细胞群比来自亲代肿瘤的细胞更具转移性。比较了几个个体亲本肿瘤衍生和肺转移衍生克隆的自发转移潜能、细胞培养形态、原发植入和继发转移部位的组织学结构以及体内和体外生长特征。这些肿瘤和细胞特性与转移潜力之间没有任何相关性。具有不同自发转移特性的亲代乳腺肿瘤来源、淋巴结转移来源和肺转移来源的细胞系和克隆应可用于研究肿瘤细胞和宿主特性在淋巴和血源性转移中的作用。
With the use of the rat 13762 mammary adenocarcinoma tumor, an animal model for spontaneous mammary tumor metastasis was developed. The parental tumor implanted sc into the mammary fat pads of female Fischer 344 rats metastasized at low frequency to lymph nodes and lung and did not metastasize to other organs. Oophorectomy did not modify the metastatic properties of the parental 13762 tumor. Cell lines were adapted to tissue culture from lymph node or lung metastases, and these were compared to parental tumor transplants in spontaneous metastasis assays. Only the cell lines established from metastases were spontaneously metastatic within 23 days after sc implantation, indicating that tumor cell populations from metastases are more metastatic than are cells from the parental tumor. Several individual parental tumor-derived and lung metastasis-derived clones were compared for spontaneous metastatic potentials, cell culture morphologies, histologic structures at primary implant and secondary metastatic sites, and growth characteristics in vivo and in vitro. There was no correlation between any of these tumor and cellular properties and metastatic potential. The parental mammary tumor-derived, lymph node metastasis-derived, and lung metastasis-derived cell lines and clones with differing spontaneous metastatic properties should prove useful in studies on the roles of tumor cell and host properties in lymphatic and blood-borne metastasis.