The conserved mitochondrial genomes of Drosophila mercatorum (Diptera: Drosophilidae) with different reproductive modes and phylogenetic implications
The conserved mitochondrial genomes of Drosophila mercatorum (Diptera: Drosophilidae) with different reproductive modes and phylogenetic implications
复制标题
具有不同生殖模式和系统发育意义的果蝇（双翅目：果蝇科）保守的线粒体基因组
DOI：
10.1016/j.ijbiomac.2019.07.184
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发表时间：
2019-10-01
影响因子：
8.2
通讯作者：
Li, Hu
中科院分区：
文献类型：
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作者：
Wang, Antong;Du, Zhenyong;Li, Hu
果蝇(果蝇科：果蝇)在日常生活中普遍存在，长期以来一直被用作生物学研究的模式生物。Mercatorum果蝇是果蝇属的重要成员之一，被用来研究细胞的中心体组装。在本研究中，我们测序和分析了斑点石藻的线粒体基因组(有丝分裂组)，发现它包含37个基因的典型结构和一个控制区。线粒体基因的排列与其他果蝇物种的排列一致，被认为是昆虫有丝分裂体的祖先组织。对23种果蝇进行了系统发育分析。我们的结果支持果蝇和苦参两个单系亚属，除了被认为是果蝇的早期分支的威利斯通果蝇。支持((D.Yakuba+D.erecta)+D.Blackogaster)拓扑结构。我们进一步比较了单性生殖和有性生殖品系的有丝分裂组。然而，仅在COI基因上发现了一个同义突变，这表明有丝分裂基因组的进化与该物种不同的生殖方式没有很强的相关性。综上所述，我们的结果表明，有丝分裂组是一个有效的分子标记，可以进一步用于果蝇和其他生物的系统发育研究。(C)2019爱思唯尔B.V.保留所有权利。
Fruit flies (Drosophilidae: Drosophila) are commonly found in daily life and have long been used as model organisms in biology researches. Drosophila mercatorum is one important member of the Drosophila genus and has been used to study centrosome assembly of cells. In this study, we sequenced and analyzed the mitochondrial genome (mitogenome) of D. mercatorum, finding that it contains the typical structure of 37 genes and a control region. The arrangement of mitochondrial genes is in accordance with that in other Drosophila species, which is considered the ancestral organization of insects' mitogenomes. Phylogenetic analyses were performed based on 23 species of Drosophila. Our results supported two monophyletic subgenera, Drosophila and Sophophora, except for D. willistoni which was presented as an early offshoot of Drosophila. The topology ((D. yakuba + D. erecta) + D. melanogaster) was supported. We further compared the mitogenomes of parthenogenesis and sexual reproduction strains of D. mercatorum. However, only one synonymous mutation in COI gene was identified, indicating mitogenomic evolution is not strongly correlated with the different reproductive modes of this species. Taken together, our results demonstrate that mitogenome is an effective molecular marker that can be further used in phylogenetic studies of Drosophila and other organisms. (C) 2019 Elsevier B.V. All rights reserved.