HEMAGGLUTINATION STUDIES WITH FORMALINIZED ERYTHROCYTES. EFFECT OF BIS-DIAZO-BENZIDINE AND TANNIC ACID TREATMENT ON SENSITIZATION BY SOLUBLE ANTIGEN.
HEMAGGLUTINATION STUDIES WITH FORMALINIZED ERYTHROCYTES. EFFECT OF BIS-DIAZO-BENZIDINE AND TANNIC ACID TREATMENT ON SENSITIZATION BY SOLUBLE ANTIGEN.
复制标题
形式化红细胞的血凝研究。
作者：
W. Butler
摘要 使用双重氮化联苯胺 (BDB) 作为偶联剂，用白喉类毒素致敏的福尔马林化红细胞将检测到 0.00015 单位/ml 的标准白喉抗毒素。因此，这样的红细胞至少与通过单宁酸法致敏的新鲜或福尔马林化的红细胞一样敏感。它们可以冷冻保存，并可以随时解冻使用。反应混合物中 BDB 的浓度至关重要。相比之下，最佳致敏作用发生在较宽的白喉类毒素浓度范围内，并且所需的抗原比之前通过 BDB 方法致敏非福尔马林化红细胞所需的抗原少 100 倍。偶联过程在 37°C 下 5 至 10 分钟内完成。讨论了与福尔马林化红细胞的制备、此类红细胞的单宁酸处理、BDB-抗原-红细胞反应混合物的制备以及血凝试验的进行相关的其他因素。
Summary Formalinized erythrocytes sensitized with diphtheria toxoid using bisdiazotized benzidine (BDB) as a coupling agent, will detect 0.00015 units/ml of standard diphtheria antitoxin. Therefore, such erythrocytes are at least as sensitive as fresh or formalinized erythrocytes sensitized by the tannic acid method. They can be stored frozen, and thawed at any time for use. The concentration of BDB in the reaction mixture is crucial. In contrast, optimal sensitization occurs over a wide range of diphtheria toxoid concentration, and 100-fold less antigen is necessary than had previously been needed to sensitize nonformalinized erythrocytes by the BDB method. The coupling procedure is complete within 5 to 10 min at 37°C. Other factors related to the preparation of formalinized erythrocytes, tannic acid treatment of such erythrocytes, preparation of the BDB-antigen-erythrocyte reaction mixture, and performance of the hemagglutination test are discussed.