Interaction of human peripheral blood monocytes with apoptotic polymorphonuclear cells
Interaction of human peripheral blood monocytes with apoptotic polymorphonuclear cells
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DOI：
10.1111/j.1365-2567.2009.03087.x
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发表时间：
2009-09-01
期刊：
影响因子：
6.4
通讯作者：
Pryjma, Juliusz R.
中科院分区：
文献类型：
--
作者：
Mikolajczyk, Tomasz P.;Skrzeczynska-Moncznik, Joanna E.;Pryjma, Juliusz R.
P>；巨噬细胞具有识别凋亡的中性粒细胞并吞噬它们的潜力，而单核细胞的同样功能尚不确定。事实上，早期的发现表明，单核细胞在分化为巨噬细胞的第三天就开始吞噬中性粒细胞。在这里，我们使用流式细胞仪和共聚焦显微镜显示，外周血单核细胞吞噬凋亡，但不是新鲜分离的粒细胞。对凋亡细胞的识别主要与CD16(+)单核细胞(CD14(高)CD16(+)和CD14(低)CD16(+))有关，需要CD36。多形核白细胞对凋亡的清除似乎独立于CD14机制。单核细胞摄取凋亡的Jurkat T细胞依赖于CD14和CD36。含有磷脂酰-L-丝氨酸的脂质体减少了与凋亡的多形核白细胞的结合。脂多糖激活的单核细胞亚群在与凋亡细胞接触时产生更多的抗炎细胞因子IL-10，而促炎细胞因子、肿瘤坏死因子-α和IL-1β的产生减少。
P>Macrophages have the potential to recognize apoptotic neutrophils and phagocytose them while the same function for monocytes is uncertain. In fact, early findings indicated that monocytes started to phagocytose neutrophils on the third day of differentiation to macrophages. Here we show, using flow cytometry and confocal microscopy, that peripheral blood monocytes phagocytose apoptotic but not freshly isolated granulocytes. Recognition of apoptotic cells is predominantly connected with CD16(+) monocytes (CD14(high) CD16(+) and CD14(dim) CD16(+)) and requires CD36. Clearance of apoptotic polymorphonuclear leucocytes appears to be independent of the CD14 mechanism. Uptake of apoptotic Jurkat T cells by monocytes is CD14 and CD36 dependent. Liposomes containing phosphatidyl-l-serine reduce binding of apoptotic polymorphonuclear leucocytes. Lipopolysaccharide-activated subpopulations of monocytes while in contact with apoptotic cells produce more anti-inflammatory cytokine interleukin-10 whereas the production of pro-inflammatory cytokines, tumour necrosis factor-alpha and interleukin-1 beta is reduced.