SEQUENCE OF DICTYOSTELIUM DIRS-1 - AN APPARENT RETROTRANSPOSON WITH INVERTED TERMINAL REPEATS AND AN INTERNAL CIRCLE JUNCTION SEQUENCE
SEQUENCE OF DICTYOSTELIUM DIRS-1 - AN APPARENT RETROTRANSPOSON WITH INVERTED TERMINAL REPEATS AND AN INTERNAL CIRCLE JUNCTION SEQUENCE
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DOI：
10.1016/0092-8674(85)90016-9
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发表时间：
1985-01-01
期刊：
影响因子：
64.5
通讯作者：
LODISH, HF
中科院分区：
文献类型：
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作者：
CAPPELLO, J;HANDELSMAN, K;LODISH, HF
盘基网柄藻转座子DIRS-2含有长末端重复序列，这些重复序列是反向的（ITR）和不相同的。我们在这里显示，内部序列包含4158个核苷酸，编码三个开放阅读框架（ORF）。两个ORF（ORF 2和3）共线，重叠超过2000个碱基。讨论了重叠区中两个DIRS-1元件之间不寻常的序列保守性。保守的阅读框架（ORF 3）包含200个氨基酸的区域，与DIRS-1具有显著的同源性，我们将DIRS-1分类为可能的反转录转座子，并提出了一个模型，通过该模型，近基因组长度的4.5 kb DIRS-1 RNA可以用于产生具有不相同反向末端重复序列的DIRS-1的基因组DNA拷贝。
The Dictyostelium discoideum transposon DIRS-2 contains long terminal repeats that are inverted (ITRs) and nonidentical. We show here that the internal sequence contains 4158 nucleotides and encodes three open reading frames (ORFs). Two of the ORFs (ORFs 2 and 3) are colinear and overlap for more than 2000 bases. Unusual sequence conservation between the two DIRS-1 elements in the overlap region is discussed. The conserved reading frame (ORF3) contains a 200 amino acid region that bears significant homology to, we classify DIRS-1 as a possible retrotransposon and propose a model by which the nearly genomic length 4.5 kb DIRS-1 RNA could be used to generate a genomic DNA copy of DIRS-1 with nonidentical inverted terminal repeats.