The effects of inulin, dried Jerusalem artichoke tuber and a multispecies probiotic preparation on microbiota ecology and immune status of the large intestine in young pigs
The effects of inulin, dried Jerusalem artichoke tuber and a multispecies probiotic preparation on microbiota ecology and immune status of the large intestine in young pigs
复制标题
DOI：
10.1080/1745039x.2016.1184368
复制
发表时间：
2016-01-01
影响因子：
2
通讯作者：
Skomial, Jacek
中科院分区：
文献类型：
--
作者：
Barszcz, Marcin;Taciak, Marcin;Skomial, Jacek
该研究旨在确定两种类型的益生元和多物种益生菌对仔猪大肠中微生物群活性和组成以及粘膜免疫的影响。将48头仔猪分为6组（n=8），从出生后第10天开始接受未添加益生菌（PU）或添加益生菌（PS）的日粮。益生菌以0.5g/kg饮食添加，并且含有：乳酸乳球菌、分歧肉杆菌、干酪乳杆菌、植物乳杆菌和酿酒酵母。PU和PS饲料配制为不添加益生元（对照）或添加2%的来自菊苣根的菊粉（IN）或4%的干燥耶路撒冷朝鲜蓟块茎（DJA）。喂食40天后，从盲肠和结肠的三个部分采集消化物和组织样本，用于分析微生物群活性和组成、分泌性免疫球蛋白A（sIgA）和上皮内淋巴细胞（IEL）。与对照组相比，IN饮食降低了盲肠直肠pH值和β-葡萄糖苷酶活性，但增加了丙酸、戊酸和总短链脂肪酸（SCFA）浓度。喂食DJA饮食增加盲肠戊酸水平，降低结肠中异酸的浓度，降低中结肠中β-葡萄糖苷酶和β-葡萄糖醛酸酶的活性，并增加双歧杆菌属。近端和远端结肠中的群体。PS饲料增加盲肠乙酸和总SCFA水平，梭菌属。远端结肠中的人群。益生菌和益生元均不影响大肠中sIgA水平或IEL数量。总之，DJA比IN更大程度地改变了仔猪大肠中的微生物群生态，并且所施用的益生菌没有增强益生元的效果。
The study aimed at determining the effect of two types of prebiotics and a multispecies probiotic on microbiota activity and composition, as well as mucosal immunity in the large intestine of young pigs. In total 48 piglets were divided into 6 groups (n=8), which received from day 10 of life probiotic-unsupplemented (PU) or probiotic-supplemented (PS) diets. Probiotics were added at 0.5g/kg diet and contained: Lactococcus lactis, Carnobacterium divergens, Lactobacillus casei, Lactobacillus plantarum and Saccharomyces cerevisiae. The PU and PS diets were formulated without prebiotic addition (control) or with addition of 2% of inulin from chicory root (IN) or 4% of dried Jerusalem artichoke tubers (DJA). After 40days of feeding, digesta and tissue samples were taken from the caecum and three sections of the colon for analyses of microbiota activity and composition, secretory immunoglobulin A (sIgA) and intraepithelial lymphocytes (IEL). IN diets decreased the caecal digesta pH and -glucosidase activity but increased propionic, valeric and total short chain fatty acid (SCFA) concentrations compared to control diets. Feeding DJA diets increased caecal valeric acid level, decreased the concentration of isoacids in the colon, reduced -glucosidase and -glucuronidase activity in the middle colon and increased Bifidobacterium spp. populations in the proximal and distal colon. PS diets increased the caecal acetic acid and total SCFA level, and Clostridium spp. populations in the distal colon. Neither probiotic nor prebiotics affected sIgA level or IEL number in the large intestine. In conclusion, DJA modified the microbiota ecology in the large intestine of young pigs to a greater extent than IN and the applied probiotic did not enhance effects of prebiotics.