SEX-DIFFERENCES IN AND EFFECTS OF ESTROGEN ON OXYTOCIN RECEPTOR MESSENGER-RIBONUCLEIC-ACID EXPRESSION IN THE VENTROMEDIAL HYPOTHALAMUS
SEX-DIFFERENCES IN AND EFFECTS OF ESTROGEN ON OXYTOCIN RECEPTOR MESSENGER-RIBONUCLEIC-ACID EXPRESSION IN THE VENTROMEDIAL HYPOTHALAMUS
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DOI：
10.1210/en.136.1.27
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发表时间：
1995-01-01
期刊：
影响因子：
4.8
通讯作者：
DORSA, DM
中科院分区：
文献类型：
--
作者：
BALE, TL;DORSA, DM
本研究应用原位杂交技术检测大鼠下丘脑催产素受体（OR）基因的表达。结合研究已经将OR定位于不同的脑区，并在下丘脑腹内侧（VMH）检测到高密度的受体，VMH是雌激素作用的记录靶点。本研究旨在比较雌、雄大鼠VMH中OR信使RNA（mRNA）的水平，并研究雌激素治疗对雌性大鼠VMH中OR mRNA水平的影响。从基因组睾丸文库中克隆大鼠OR基因后，生成探针用于原位杂交测定，以评估VMH中OR mRNA表达的性别差异。此外，卵巢切除的女性与雌激素治疗，VMH或mRNA的表达进行了比较，在卵巢切除或完整的女性。这些研究的结果表明，雄性大鼠VMH中OR mRNA的表达水平高于雌性大鼠。雌激素治疗的卵巢切除的女性表现出显着更大的表达VMH比油加热或完整的女性。这些结果支持结合研究表明，催产素结合VMH调节性腺类固醇，并表明雌激素可能直接或间接调节OR基因的转录。
In situ hybridization techniques were used in the present study to detect hypothalamic expression of the oxytocin receptor (OR) gene. Binding studies have localized OR to Various brain regions and have detected a high density of receptors in the ventromedial hypothalamus (VMH), a documented target of estrogen action. This study was designed to compare levels of OR messenger RNA (mRNA) in the VMH of male and female rats and to study the effects of estrogen treatment an mRNA levels in the VMH of female rats. After cloning a rat OR gene from a genomic testes library, a probe was generated for use in in situ hybridization assays to evaluate sex differences in OR mRNA expression in the VMH. In addition, ovariectomized females were treated with estrogen, and VMH OR mRNA expression was compared with that in ovariectomized or intact females. The results of these studies showed that male rats expressed higher levels of OR mRNA in the VMH than females. Estrogen-treated ovariectomized females exhibited significantly greater expression in the VMH than either oil-heated or intact females. These results support binding studies that have shown oxytocin binding in the VMH to be regulated by gonadal steroids and suggest that estrogen may either directly or indirectly regulate transcription of the OR gene.